Metabolomics is an important tool for the evaluation of the human condition, in both health or disease. This study analyzed the salivary components of type I diabetic children (DM1) under six years of age, to assess oral health related to diabetes control, as well as metabolite profiling using NMR. Partial least squared discriminant analysis (PLS-DA) was used to compare healthy (HG) and uncontrolled DM1 subjects that demonstrated a separation between the groups with classificatory performance of ACC = 0.80, R(2) = 0.92, Q(2) = 0.02 and for DM1 children with glycemia >200 mg/dL of ACC = 0.74, R(2) = 0.91, Q(2) = 0.06. The metabolites that mostly contributed to the distinction between the groups in the loading factor were acetate, n-acetyl-sugar, lactate, and sugar. The univariate analysis showed a decreased salivary concentration of succinic acid and increased levels of lactate, acetate, and sucrose in uncontrolled and DM1 children with glycemia >200 mg/dL. The present study demonstrates that the salivary profile of DM1 differs from that of HG children. It appears that diabetes status control has an important effect on the salivary composition.

Palavras-chave: Traumatismo. DentesPermanentes. Fratura Coronária Complicada. Qualidade de Vida. RESUMOObjetivo: O objetivo do estudo foi relatar o manejo de duas fraturas complicadas de incisivos permanentes e como o tratamento destas lesões dentárias influencia a qualidade de vida relacionada à saúde bucal (QHRQoL). Relato de caso: No primeiro caso, foi realizada pulpectomia devido necrose pulpar e rizogênese completa. No segundo caso, a pulpotomia foi realizada, pois, a polpa apresentava vitalidade e o dente rizogênese incompleta. A colagem dos fragmentos foi realizada em ambos os casos. QHRQoL foi avaliada antes e uma semana após o tratamento. No caso 1, os escores variaram de 32 a 9 no CPQ 11-14 , de 42 a 12 no P-CPQ e de 24 a 4 no FIS. No caso 2, os escores variaram de 38 a 20 no CPQ 8-10 , de 94 a 28 no P-CPQ e de 39 a 10 no FIS. Conclusão: A colagem de fragmentos é uma solução eficiente, assim como a terapia endodôntica. Além disso, o tratamento do traumatismo dentário promoveu uma melhora na qualidade de vida das crianças e suas famílias, e podendo ser observado uma semana após o tratamento.

This study compares the oral health, dietary and oral hygiene habits and dental care experience of children with type 1 diabetes (DG) to healthy ones (HG) under six years old. Methods: The children were chosen matched by gender and age in each group. Diabetes status from DG was determined using HbA1c criteria levels: <8.5% (69 mmol/mol) was considered controlled and >8.5% uncontrolled. Guardians were asked about oral hygiene, dietary habits and dental care experience. Oral examination was performed in order to assess dental caries (dmft), plaque and calculus indexes, as well as oral manifestations in soft tissues. Data were described and analyzed by SPSS 20.0 software through Chi-square and T-tests. Results: A total of 68 children were enrolled in this study. Children with diabetes differed from healthy ones in relation to geographic tongue, breath acetone and xerostomia (p<0.05). Only 23% (n=7) of DG were considered as presenting uncontrolled diabetes status. Statistical differences between controlled and uncontrolled diabetic status were observed among children, such as age at diagnosis, disease duration, sugar consumption, dental visits, type of experience during dental appointment, presence of geographic tongue, dental caries, reported breath acetone and xerostomia (p<0.05). Toothache was the main reason that DG children had been to a dentist before. Conclusion: Oral health and dietary habits of children with diabetes differed from healthy ones. Most children with diabetes had never been to a dentist before. Oral manifestation and oral habits diverged from children uncontrolled and controlled diabetes.

OBJECTIVE: aimed to compare three saliva collection methods in terms of their acceptability by young children, the salivary samples were submitted to total protein concentration measurement and a SDS-Page gel electrophoresis. METHODS: The study was composed of 34 healthy children with complete primary dentition. They were selected from those who presented themselves for dental treatment at the Dental School of a public university in Rio de Janeiro, Brazil. Anamnesis was performed, followed by the saliva collection and intra-oral examination for the evaluation of dmf-t index (WHO). After all the salivary collection methods were performed, children were asked about which method of collection they would prefer. RESULTS: When asked about the preferred method of saliva collection, 50% of children answered that they prefer to spit into a tube, while 37.5% had chosen using Salivette® collection device and 12.5% had opted by using the pipette. CONCLUSION: The collection by spitting saliva into a tube can be recommend for saliva collection in young children once the method is well accept by them and it is not necessary additional cost.