As the most common post-transcriptional RNA modification, m6A methylation extensively regulates the structure and function of RNA. The dynamic and reversible modification of m6A is coordinated by m6A writers and erasers. m6A reader proteins recognize m6A modification on RNA, mediating different downstream biological functions. mRNA m6A modification and its corresponding regulators play an important role in cancers, but its characteristics in the precancerous stage are still unclear. In this study, we used oral precancerous DOK cells as a model to explore the characteristics of transcriptome-wide m6A modification and major m6A regulator expression in the precancerous stage compared with normal oral epithelial cell HOEC and oral cancer cell SCC-9 through MeRIP-seq and RT-PCR. Compared with HOEC cells, we found 1180 hyper-methylated and 1606 hypo-methylated m6A peaks and 354 differentially expressed mRNAs with differential m6A peaks in DOK cells. Although the change of m6A modification in DOK cells was less than that in SCC-9 cells, mRNAs with differential m6A in both cell lines were enriched into many identical GO terms and KEGG pathways. Among the 20 known m6A regulatory genes, FTO, ALKBH5, METTL3 and VIRMA were upregulated or downregulated in DOK cells, and the expression levels of 10 genes such as METTL14/16, FTO and IGF2BP2/3 were significantly changed in SCC-9 cells. Our data suggest that precancerous cells showed, to some extent, changes of m6A modification. Identifying some key m6A targets and corresponding regulators in precancerous stage may provide potential intervention targets for the prevention of cancer development through epigenetic modification in the future.
Zirconium alloys remain as the main cladding materials in most water reactors. CZ zirconium alloy is recently developed for advanced PWR fuel assembly (STEP) designed by China General Nuclear Power Group (CGN). There are two kinds of zirconium alloys, designated as CZ1 and CZ2 respectively. It is well known that creep property is one of the most important characteristics to evaluate the integrated performance of new commercial alloys. In this study, we have collected the creep date of CZ alloys heat-treated at different temperatures. The result shows that the creep rates of the zirconium alloys enlarge with the creep stress in the temperature of 375 °C. The creep resistance is improved with the increasing annealing temperature. The creep resistance of CZ-RXA (high temperature) is better than CZ-SRA (low temperature). The creep rate of CZ alloys at relatively lower annealing temperature is much larger than that at higher annealing temperature. At the given stress condition, the creep behaviors of different annealed CZ alloys are found to have almost the same tendency. In addition, the creep rate of CZ1 is smaller than CZ2 at the same annealing temperature.
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