Griffithsin is a lectin with potent antiviral activity against enveloped viruses. The objective of this study was to assess Griffithsin's inhibitory effect on porcine epidemic diarrhea virus (PEDV). The results showed that Griffithsin reduced PEDV infection of Vero cells by approximately 82.8%. Moreover, using time-of-addition assays and RT-qPCR, we found that delayed addition of Griffithsin had a weaker inhibitory effect on PEDV than earlier treatment. The mechanism of Griffithsin's action against PEDV involved both preventing viral attachment to host cells and disrupting cell-to-cell transmission; its dual mode of action distinguished Griffithsin from most other antiviral drugs. In conclusion, Griffithsin was identified as a potent PEDV inhibitor and may represent a candidate drug for preventing PEDV infection.
Herein, we reported a Ni-catalyzed
carbonylation of cyclopropanol
with benzyl bromide to afford multisubstituted cyclopentenone under
1 atm of CO. The reaction proceeds through cascade carbonylation of
benzyl bromides, followed by generation of nickel homoenolate from
cyclopropanols via β-C elimination to afford 1,4-diketones,
which undergoes intramolecular Aldol condensation to furnish highly
substituted cyclopentenone derivatives in moderate to good yields.
The reaction exhibits high functional group tolerance with broad substrate
scope.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.