The immunopotentiator CVC1302 enhances immune efficacy and protective ability of foot-and-mouth disease virus vaccine in pigs

Enhanced Immune Responses with Serum Proteomic Analysis of Hu Sheep to Foot-and-Mouth Disease Vaccine Emulsified in a Vegetable Oil Adjuvant

“…Serum FMDV-specific antibody titers were determined by a liquid phase blocking (LPB) ELISA kit (Lanzhou Veterinary Research Institute, Lanzhou, China) according to the manufacturer’s instructions [ 2 , 36 ]: LPB-ELISA antibody titers ≥ 7 log2 (1:128) were considered to have protection ( Figure S1 ). Briefly, 50 µL of two-fold serial dilutions of serum samples and 50 µL of FMDV antigen (1:20 dilution) were added to a U-bottomed 96-well plate and incubated for 1.5 h at 37 °C.…”

“…Serum FMDV-specific antibody titers were determined by a liquid phase blocking (LPB) ELISA kit (Lanzhou Veterinary Research Institute, Lanzhou, China) according to the manufacturer's instructions [2,36]: LPB-ELISA antibody titers ≥ 7 log2 (1:128) were considered to have protection ( Figure S1). Briefly, 50 µL of two-fold serial dilutions of serum samples and 50 µL of FMDV antigen (1:20 dilution) were added to a U-bottomed 96-well plate and incubated for 1.5 h at 37 • C. The mixtures then were transferred into a 96-well ELISA plate precoated with rabbit anti-FMDV polyclonal antibody and incubated for 1 h at 37 • C. After five washes, plates were incubated with 50 µL of guinea pig antiserum against FMDV O serotype for 30 min at 37 • C. Then, 50 µL of rabbit anti-guinea pig IgG/HRP was added to the 96-well plate after a washing step (total of five washes with PBST) and incubated for 30 min at 37 • C. The plate was washed and 50 µL of the substrate/chromophore mixture was added to each well, and the plate was incubated for 15 min at 37 • C in the dark.…”

“…The Reed and Muench formula was adopted to calculate of SN titer [38]. Results were expressed as the Log 10 SN titer [2]. Sera with titers > 1.5 Log 10 are considered positive (OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2019) [17].…”

“…Foot-and-mouth disease (FMD) is a highly contagious viral disease that spreads rapidly among cloven hoofed animals, causing large economic losses after outbreaks [1][2][3][4]. The disease is caused by was the product of Seppic Co. Ltd. (Shanghai, China).…”

“…Foot-and-mouth disease (FMD) is a highly contagious viral disease that spreads rapidly among cloven hoofed animals, causing large economic losses after outbreaks [ 1 , 2 , 3 , 4 ]. The disease is caused by the FMD virus (FMDV), which includes seven serotypes (A, C, O, South African Territories (SAT) 1, SAT 2, SAT 3, and Asia 1) and multiple subtypes within each serotype [ 5 ].…”