Diaper dermatitis is a common cutaneous disorder characterized with red and spotty, and is sometimes itchy in the diaper area of the infant. The condition is one type of acute nonimmunologic skin inflammation. There were mainly three types of diaper dermatitis, including diaper dermatitis caused by candidiasis, irritant contact dermatitis, and chaffing dermatitis. 1 Chaffing is the most widespread form of mild diaper dermatitis commonly affecting most infants at some point in time. It happens in areas where frictional coefficient from the nappy is high, including the inner surface of the buttocks, thighs, and the surface of the genital areas. 1 Irritant contact diaper dermatitis(IDD) is commonly found in the gluteal crease, buttocks, perianal, and the public areas. It can also include the lower abdominal area as well as the upper thigh area. Irritant contact diaper dermatitis can range in severity from a mild case, with mild localized erythema and minimal scaling, to a moderate case, with increased erythema as well as papules noted to the affected area, and finally to a more severe case where the skin in the diaper area is noted to have papules, pustules, and skin breakdown with open areas. 2 Abstract Background: Diaper dermatitis (DD) is the most common acute inflammatory skin disease. It has a serious effect on children's and families' quality of life. We aimed to screen and evaluate the efficacy of different formulas for relieving the diaper dermatitis symptoms by developing a kind of diaper dermatitis-like reconstructed human skin equivalent in vitro. Materials and Method: We developed the human skin equivalent for diaper dermatitis with 0.2% Sodium lauryl sulfate (SLS). The diaper dermatitis-like human skin equivalent was characterized by high level of inflammation, such as overexpression of interleukin-1α (IL-1α), and impaired skin barrier. Four formulas with potential of antiinflammation and promotion of skin barrier function were topically applied on the diaper dermatitis-like human skin equivalent surface. The afterward protection efficacy was evaluated by endpoints of IL-1α, tissue viability, and skin barrier function. Results: The chemical irritant induced high release of IL-1α, impaired tissue viability, and skin barrier function. The cream prepared with potential of anti-inflammation and skin protection could effectively decrease and relive the impact of irritant with decreased level of IL-1α and the higher tissue viability than the placebo exposure. Conclusion: The results showed that diaper dermatitis-like human skin equivalent induced by SLS can mimic the skin irritation response of the diaper rash. K E Y W O R D S anti-inflammation, Diaper dermatitis, reconstructed human skin equivalent in vitro, skin barrier How to cite this article: Zhuang L, Gu H, Huang Y, Li X, Lu Y, Kaku K. Development of a new diaper dermatitis-like reconstructed skin equivalent for testing children atopic dermatitis relieving cosmetics. Skin Res Technol.

Quarantine is one of the effective approaches to control the spread of COVID-19. However, prolonged isolation may harm the health of residents, especially students, who are quarantined in dormitories. This study surveyed students’ behaviors, living environment, and psychological state through an online questionnaire. The results showed that during the isolation period, the participants’ discomfort mainly came from high temperatures, high humidity, loud sounds, and dark lighting. Moreover, about half of the students experienced severe anxiety or depression, with poor sleep and an unclear mind being the most common problems. Additionally, this study focused on exploring the correlations between environmental perceptions and the psychological state of isolated students, that were identified to be significant. Furthermore, as isolation time was prolonged, the psychological state of isolated students worsened, and the most significant factor changed from thermal sensations to acoustic sensations. Interestingly, the psychological state of males was worse than females, and they were more easily affected by their thermal and acoustic environments. Therefore, keeping the environment comfortable has a positive influence on maintaining the good psychological condition of people in isolation. This study can provide suggestions for the improvement of indoor environments and for the mental health of people in isolation and in other similar situations.