<p>Introduction<br />Skin hydration decreases with aging. Aquaporin-3 (AQP3) is a major protein that plays a role in skin hydration, therefore it is a novel target for skin moisturizing treatment. Retinoic acid (RA) as a well-known active agent in antiaging treatment increases AQP3 expression, but frequently causes harmful side effects. Asiaticoside, a saponin compound isolated from Centella asiatica (CA) is also known as an antiaging cosmetic and plays a role in wound healing. The aim of this study was to evaluate and compare the effect of asiaticoside isolated from CA and the effect of RA on the AQP3 expression in normal human epidermal keratinocytes (NHEKs).</p><p>Methods <br />An experimental laboratory study was performed using primary NHEKs that were derived from the foreskin of a boy. AQP3 expression in NHEKs was examined in vitro after the cells were incubated for 24 hours with asiaticoside or with RA at several concentrations. The AQP3 expression was evaluated by immunocytochemistry and quantitatively analyzed by Image-J software. Independent t-test and one-way ANOVA were used to analyze the data, followed by post-hoc Tukey test.</p><p>Results<br />There was an increasing trend of AQP3 expression upon exposure to asiaticoside at all concentrations compared to the control group. However, RA exposure seemed to induce a higher level of AQP3 expression. Asiaticoside effected a lower increase in AQP3 expression in NHEKs than did RA (p=0.042). Optimal results were achieved at 1 mg/mL concentration of asiaticoside.</p><p>Conclusions<br />Asiaticoside isolated from CA can enhance the AQP3 expression in NHEKs. Therefore it can be used as an active ingredient in cosmetic moisturizer formulation for dry skin treatment.</p>
Kelompok protein Aquaporins (AQPs) dikenal sebagai saluran air dan gliserol untuk memfasilitasi transportasi air dan gliserol yang melintasi membran sel dan juga memiliki peranan dalam pemeliharaan kelembaban lapisan epidermis. Aquaporin 3 (AQP3) adalah bagian dari aquaglyceroporin dan banyak terdapat pada membran plasma keratin pada lapisan epidermis kulit. Ekspresi AQP3 tampak dalam sel keratinosit dan fibroblas kulit. AQP3 terlihat pada lapisan basal dari epidermis kulit, spinosum dan jaringan fibroblast kulit dibagian dermis kulit. Tanda terjadinya penuaan kulit adalah keringnya kulit, peningkatkan kerapuhan kulit, penurunan elastisitas dan penyembuhan luka yang tertunda. AQP3 adalah protein utama yang mempengaruhi hidrasi kulit dan menurun pada penuaan kulit. AQP3 menjadi protein kunci untuk target pengobatan masa depan terhadap penuaan kulit (kulit kering), sehingga mulai banyak dibuat pelembab yang dapat mengekspresikan protein AQP3 The protein family of Aquaporins (AQPs) known as water and glycerol channels to facilitate the transport of water and glycerol across cell membrane and also have the role in epidermal water maintenance. Aquaporins-3 (AQP3) is a member of aquaglyceroporin and the must abundant AQP3 present in the skin, in plasma membrane of epidermal cells. AQP3 showed expression in skin keratinocyte in basal layer of epidermis and spinosum, and in fibroblast skin dermis. A feature of skin aging is dry skin, increase skin fragility, decrease elasity and delayed wound healing. AQP3 is a major protein implicated in skin hydration and decrease with skin aging therefor AQP3 appears to be a key protein as a target and potential target for drug development for the future treatment of skin aging (dry skin), as a novel moisturizer.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.