Many organisms lose the capacity to regenerate damaged tissues as they mature. Damaged Drosophila imaginal discs regenerate efficiently early in the third larval instar (L3) but progressively lose this ability. This correlates with reduced damage-responsive expression of multiple genes, including the WNT genes wingless (wg) and Wnt6. We demonstrate that damage-responsive expression of both genes requires a bipartite enhancer whose activity declines during L3. Within this enhancer, a damage-responsive module stays active throughout L3, while an adjacent silencing element nucleates increasing levels of epigenetic silencing restricted to this enhancer. Cas9-mediated deletion of the silencing element alleviates WNT repression, but is, in itself, insufficient to promote regeneration. However, directing Myc expression to the blastema overcomes repression of multiple genes, including wg, and restores cellular responses necessary for regeneration. Localized epigenetic silencing of damage-responsive enhancers can therefore restrict regenerative capacity in maturing organisms without compromising gene functions regulated by developmental signals.DOI: http://dx.doi.org/10.7554/eLife.11588.001

The study of regeneration in Drosophila imaginal discs provides an opportunity to use powerful genetic tools to address fundamental problems pertaining to tissue regeneration and cell plasticity. We present a historical overview of the field and describe how the application of modern methods has made the study of disc regeneration amenable to genetic analysis. Discs respond to tissue damage in several ways: (a) Removal of part of the disc elicits localized cell proliferation and regeneration of the missing tissue. (b) Damage at specific locations in the disc can cause cells to generate disc-inappropriate structures (e.g., wing instead of leg), a phenomenon known as transdetermination. (c) Diffuse damage to imaginal discs, results in compensatory proliferation of surviving cells. Candidate-gene approaches have implicated the JNK, Wingless, and Hippo pathways in regeneration. Recently developed systems will enable extensive genetic screens that could provide new insights into tissue regeneration, transdetermination and compensatory proliferation.

SUMMARYTwo types of information are particularly valuable in understanding the development of a tissue or an organ from a small population of founder cells. First, it is useful to know the composition of the final structure in terms the contribution of individual founder cells. Second, it is important to understand cell-cell interactions. To facilitate the study of both of these aspects of organ development at a tissue-wide level, we have developed a method, TIE-DYE, that allows simultaneous lineage tracing of multiple cell populations as well as the genetic manipulation of a subset of these populations. Seven uniquely marked categories of cells are produced by sitedirected recombination of three independent cassettes. We have used the TIE-DYE method to estimate the number of founder cells that give rise to the wing-imaginal disc during normal development and following compensatory growth caused by X-ray irradiation of the founder cells. We also show that four out of the seven types of marked clones can be genetically manipulated by gene overexpression or RNAi knockdown, allowing an assessment of the consequences of these manipulations on the entire wing disc. We demonstrate the utility of this system in studying the consequences of alterations in growth, patterning and cell-cell affinity.

Increased local trapping of morphogens within tissues as they grow would reduce circulating levels and can therefore provide a systemic readout of the status of their growth and maturation.

SUMMARYAlthough the liver and ventral pancreas are thought to arise from a common multipotent progenitor pool, it is unclear whether these progenitors of the hepatopancreas system are specified by a common genetic mechanism. Efforts to determine the role of Hnf1b and Wnt signaling in this crucial process have been confounded by a combination of factors, including a narrow time frame for hepatopancreas specification, functional redundancy among Wnt ligands, and pleiotropic defects caused by either severe loss of Wnt signaling or Hnf1b function. Using a novel hypomorphic hnf1ba zebrafish mutant that exhibits pancreas hypoplasia, as observed in HNF1B monogenic diabetes, we show that hnf1ba plays essential roles in regulating β-cell number and pancreas specification, distinct from its function in regulating pancreas size and liver specification, respectively. By combining Hnf1ba partial loss of function with conditional loss of Wnt signaling, we uncover a crucial developmental window when these pathways synergize to specify the entire ventrally derived hepatopancreas progenitor population. Furthermore, our in vivo genetic studies demonstrate that hnf1ba generates a permissive domain for Wnt signaling activity in the foregut endoderm. Collectively, our findings provide a new model for HNF1B function, yield insight into pancreas and β-cell development, and suggest a new mechanism for hepatopancreatic specification.

Abstract:For many organisms, developmental transitions are triggered by a neuroendocrine axis and are contingent upon multiple organs achieving sufficient growth and maturation. How the status of peripheral organs is communicated to the neuroendocrine axis is not known. In Drosophila larvae, metamorphosis is triggered by the steroid hormone ecdysone, secreted by the prothoracic gland (PG). Here we show that the BMP2/4 ortholog Dpp, which regulates growth and patterning of larval imaginal discs, also functions as a systemic signal to regulate developmental timing. Dpp from peripheral tissues, mostly imaginal discs, can reach the PG and inhibit ecdysone biosynthesis. As the discs grow, Dpp signaling decreases in the PG, thus alleviating the inhibition of ecdysone biosynthesis, and permitting entry into metamorphosis.We suggest that if a tissue can trap more morphogen locally as it grows and matures, then circulating levels of morphogen can provide a systemic readout of organ size and maturation.

Pasien stroke mengalami gangguan dalam pergerakan. Gangguan pergerakan yang dialami oleh peasien membuat pasien memiliki tingkat ketergantungan yang tinggi kepada perawat dalam memenuhi kebutuhan dasarnya. Studi ini bertujuan untuk mengetahui penatalaksanaan ketergantungan pasien stoke. Studi kasus ini menggunakan desain studi kuantitatif deskriptif dengan pendekatan asuhan keperawatan. Studi ini mengelola pasien stroke yang diperoleh melalui accidental sampling. Hasil studi menunjukkan bahwa pasien stroke hemiparese memiliki tingkat ketergantungan sedang. Pasien dengan tingkat ketergantungan sedang membutuhkan bantuan 1 orang perawat dalam setiap shift untuk  memenuhi kebutuhan dasarnya. Pemenuhan kebutuhan dasar pasien stroke dapat dibantu oleh perawat dan keluarga pasien. Pasien stroke dengan tingkat ketergantungan sedang membutuhkan perawatan selama 3 jam dalam 24 jam.

No abstract