The BMP2/4 ortholog Dpp can function as an inter-organ signal that regulates developmental timing

Setiawan, Linda; Pan, Xueyang; Woods, Alexis L; O’Connor, Michael B.; Hariharan, Iswar K.

doi:10.26508/lsa.201800216

Cited by 39 publications

(33 citation statements)

References 65 publications

(98 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Setiawan et al hypothesized that this was due in part to the dilution of circulating Dpp as a result of increased hemolymph volume and the trapping of Dpp in imaginal disc tissues, but were unable to identify how the tissues trapped Dpp (Setiawan et al, 2018).…”

Section: Regulated Maturation Of the Epithelial Barriermentioning

confidence: 99%

Ecdysone regulates theDrosophilaimaginal disc epithelial barrier, determining the duration of regeneration checkpoint delay

DaCrema

Bhandari

Karanja

et al. 2020

Preprint

View full text Add to dashboard Cite

Regeneration of Drosophila imaginal discs, larval precursors to adult tissues, produces a systemic response, a regeneration checkpoint that coordinates regenerative growth with developmental progression. This regeneration checkpoint is coordinated by the release of the relaxin-family peptide Dilp8 from regenerating tissues. Secreted Dilp8 protein can be detected within the imaginal disc lumen. The disc epithelium separates from the lumen from the larval hemolymph and the targets for Dilp8 activity in the brain and prothoracic gland. Here we demonstrate that the imaginal disc epithelial barrier limits Dilp8 signaling and checkpoint delay. We also observe that the wing imaginal disc barrier becomes more restrictive during development, becoming impermeable only at end of the final larval instar. This change in barrier permeability is driven by the steroid hormone ecdysone and correlates with changes in localization of Coracle, a component of the septate junctions that is required for the late, impermeable epithelial barrier. Based on these observations, we propose that the imaginal disc epithelial barrier regulates the duration of the regenerative checkpoint, providing a mechanism by which tissue function can signal the completion of regeneration.Summary StatementEcdysone signaling directs the Drosophila third instar imaginal disc epithelial barrier to mature, becoming more restrictive. This mature barrier limits Dilp8 signaling and determines the duration of the regeneration checkpoint.

show abstract

Section: Regulated Maturation Of the Epithelial Barriermentioning

confidence: 99%

Ecdysone regulates theDrosophilaimaginal disc epithelial barrier, determining the duration of regeneration checkpoint delay

DaCrema

Bhandari

Karanja

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Genes encoding their receptors tkv, put, and sax are expressed in AMPs, raising the possibility that Dpp could signal between these cell types. However, in contrast to Hh ligand, Dpp is known to spread widely (Harmansa et al, 2015), even beyond the disc (Setiawan et al, 2018), and thus might regulate AMP gene expression in a more widespread manner. Individual plexins and semaphorins are differentially expressed in the epithelium and the AMPs, and could potentially mediate contact-dependent signaling between the two cell types.…”

Section: Identification Of Other Ligand-receptor Interactions In the mentioning

confidence: 99%

Single-cell transcriptomics of theDrosophilawing disc reveals instructive epithelium-to-myoblast interactions

Everetts¹,

Worley²,

Yasutomi³

et al. 2020

Preprint

View full text Add to dashboard Cite

In both vertebrates and invertebrates, generating a functional appendage requires interactions between ectoderm-derived epithelia and mesoderm-derived cells. To investigate such interactions, we used single-cell transcriptomics to generate a cell atlas of the Drosophila wing disc at two time points during development. Using these data, we investigate gene expression using a multi-layered model of the wing disc and catalogued ligand-receptor pairs that could mediate signaling between epithelial cells and adult muscle precursors (AMPs). We found that localized expression of the FGF ligands, Thisbe and Pyramus, in the disc epithelium regulates the number and location of the AMPs. In addition, Hedgehog ligand from the epithelium activates a specific transcriptional program within adjacent AMP cells, which is critical for proper formation of a subset of the direct flight muscles. More generally, our annotated atlas provides a global view of potential cell-cell interactions between subpopulations of epithelial and myogenic cells.

show abstract

“…Cellular level coordination of receptor-mediated signals is very common during development. The PG is a good example of this coordination, as it integrates a large variety of signals, such as PTTH (Shimell et al, 2018), Hedgehog (Palm et al, 2013;Rodenfels et al, 2014), Activin (Gibbens et al, 2011), Bone Morphogenetic Protein (BMP) (Setiawan et al, 2018), etc., and interprets them to produce a precisely controlled amount of hormone. At least four RTKs (Torso, Egfr, Alk and Pvr) are expressed in the PG, all of which activate the Ras/Erk pathway (Cruz et al, 2020;Rewitz et al, 2009) (Figure 1B and 2C).…”

Section: Multiple Rtk Signals Coordinate In the Pg To Regulate Develomentioning

confidence: 99%

“…For instance, the PG cells respond to insulin signals reflecting the general nutritional state (Colombani et al, 2005;Mirth et al, 2005). In addition, systemic BMP signals help coordinate metamorphosis with appropriate imaginal disc growth (Setiawan et al, 2018). The PGNs in turn, receive presynaptic inputs from various upstream neurons that regulate circadian and pupation behaviors (Deveci et al, 2019;Imura et al, 2020;McBrayer et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Coordination Among Multiple Receptor Tyrosine Kinase Signals ControlsDrosophilaDevelopmental Timing and Body Size

Pan

O’Connor

2020

Preprint

Self Cite

View full text Add to dashboard Cite

Body size and the timing of metamorphosis are two important interlinked life-history traits that define holometabolous insect development. Metamorphic timing is largely controlled by a neuroendocrine signaling axis composed of the prothoracic gland (PG) and its presynaptic neurons (PGNs). The PGNs produce prothoracicotropic hormone (PTTH) that stimulates the PG to produce the metamorphosis inducing hormone ecdysone (E) through activation of Torso a Receptor tyrosine kinase the Receptor Tyrosine kinase and its downstream Ras/Erk signal transducers. Here we identify two additional timing signals produced by the RTKs Anaplastic lymphoma kinase (Alk) and the PDGF/VEGF-receptor related (PvR), Similar to Torso, both Alk and PvR trigger Ras/Erk signaling in the PG to up regulate expression of E biosynthetic enzymes, while Alk also suppresses autophagy induction after critical weight by activating Pi3K/Akt. When overexpressed, both RTKs hyperactivate an endogenous low-level Jak/Stat signal in the PG resulting in developmental delay or arrest. The Alk ligand Jelly belly (Jeb) is produced by the PGNs, and together with PTTH serves as a second PGN derived tropic factor that stimulates E production by the PG. In addition, we find that Pvf3, a PvR ligand, is also produced by the PGNs, but we show that the activation of PvR primarily relies on autocrine signaling by PG-derived Pvf2 and Pvf3. These findings illustrate that a multitude of juxtracrine and autocrine signaling systems have evolved to regulate the timing of metamorphosis, the defining event of holometabolous development.

show abstract

The BMP2/4 ortholog Dpp can function as an inter-organ signal that regulates developmental timing

Abstract: Increased local trapping of morphogens within tissues as they grow would reduce circulating levels and can therefore provide a systemic readout of the status of their growth and maturation.

Cited by 39 publications

References 65 publications

Ecdysone regulates theDrosophilaimaginal disc epithelial barrier, determining the duration of regeneration checkpoint delay

Ecdysone regulates theDrosophilaimaginal disc epithelial barrier, determining the duration of regeneration checkpoint delay

Single-cell transcriptomics of theDrosophilawing disc reveals instructive epithelium-to-myoblast interactions

Coordination Among Multiple Receptor Tyrosine Kinase Signals ControlsDrosophilaDevelopmental Timing and Body Size

Contact Info

Product

Resources

About