With the rapid development of molecular biology and biochemical technology, great progress has been made in the study of peptides. Peptides are easy to digest and absorb, with lowering of blood pressure and cholesterol, improving immunity, regulating hormones, antibacterial, and antiviral effects. Peptides also have physiological regulation and biological metabolism functions with applications in the fields of feed production and biomedical research. In the future, the research focus of bioactive peptides will focus on their efficient preparation and application. This article introduces a comprehensive review of the types, synthesis, functionalization, and bio‐related applications of bioactive peptides. For this aim, we introduced in detail various biopeptides and then presented the production methods of bioactive peptides, such as enzymatic synthesis, microbial fermentation, chemical synthesis, and others. The applications of bioactive peptides for anticancers, immune therapy, antibacterial, and other applications have been introduced and discussed. And discussed the development prospects of biologically active peptides.

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Four monodisperse porous polymer microspheres were successfully prepared by seed emulsion polymerization and used as stationary phases for HPLC and preparative high-performance liquid chromatography (Prep-HPLC). All four polymer microspheres(polystyrene–polystyrene (PS-PS), polystyrene-poly(glycidyl methylate) (PS-PGMA), polystyrene-poly(methyl methylate) and poly(glycidyl methylate)–poly(glycidyl methylate) were used for filling HPLC empty columns. According to the analysis results of the HPLC column, PS-PS and PS-PGMA microspheres were screened out as the stationary phase of Prep-HPLC. The industrial-grade phytol was successfully separated and purified, and the purity of the final phytol was as high as 99%. The two types of polymer microspheres have been applied to industrial-grade phytol purification and have been used in factories.

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