We identified 14 immune-related differentially Expressed Genes (DEGs) between COVID-19 patients and normal controls and the receiver operator characteristic curve results showed that they could be used to discriminate COVID-19 patients from healthy controls. Single-sample gene set enrichment analysis and CIBERSORT analysis displayed immune landscape of COVID-19 patients that the fraction of immune cells (like B cell subtypes and T cell subtypes) decreased distinctly in the first SARS-CoV-2 infection which may further weaken immunity of cancer patients and increasing inflammatory cells (Neutrophils and Macrophages) may further promote inflammatory response of cancer patients. Based on expression levels of 14 DEGs we found that first SARS-CoV-2 infection may accelerate progression of cancer patients by Kaplan-Meier survival, immune subtypes and tumor microenvironment analyses, and may weaken anti-PD-1 monoclonal antibody treatment effect of cancer patients by weighted gene co-expression network, tumor mutation burden and microsatellite instability analysis. The second SARS-CoV-2 infection was beneficial to control development of tumor seemingly, but it may be difficult for cancer patients to experience destroy successfully from first SARS-CoV-2 infection, let alone benefits from second SARS-CoV-2 infection. In addition, this study also emphasized significance of multi-factor analysis when analyzing impacts of SARS-CoV-2 infection on cancer patients.
Vibrio splendidus is one of the common pathogens in the ocean and infects Apostichopus japonicus, Atlantic salmon and Crassostrea gigas, leading to a variety of diseases. In this study, a virulent phage, VS1, which infects V. splendidus, was isolated from aquaculture ponds in Dalian, China. Its genome annotation and characterization were detected. The complete genome of phage VS1 consists of a linear double-stranded DNA that is 248,270 bp in length with an average G + C content of 42.5% and 144 putative protein-coding genes, and 116 genes have known functions. There are 4 tail fiber genes in the positive and negative strands of the phage VS1 genome. The domains and crystal structure of the phage VS1 tail fibers were obtained from the Protein Data Bank and SMART (http://smart.embl.de) database. The bacteriostatic effect of phage VS1 on V. splendidus culture was 93.5 times. Comparative genomic and phylogenetic analyses indicate that phage VS1 is a novel phage. This study provides the genome characterization of the novel phage VS1 that infects V. splendidus.
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