A multichain polypeptide poly-dl-alanyl-poly-l-lysine (A--L) was immunogenic in guinea pigs randomly bred and of inbred strain 2 and non-immunogenic for guinea pigs of inbred strain 13. The immune response to A--L in randomly bred guinea pigs was not related to the ability to respond to a DNP-p(Lys) conjugate.
We investigated the ability of the proto-oncogene L-myc to substitute for c-myc in blocking murine erythroleukemia differentiation. Murine erythroleukemia cells (line C19) were transfected with recombinant plasmids containing genomic and cDNA fragments of the L-myc gene driven by a Moloney murine leukemia virus long terminal repeat. Clones expressing constitutive high levels of L-myc failed to differentiate in response to the chemical inducer N,N'-hexamethylene bisacetamide (HMBA). The block to differentiation correlated with the level of L-myc expression. Furthermore, transfected clones grown in the presence of inducer for an extended period of time showed an increased level of L-myc expression. These results suggest that functional domains of the c-myc gene involved in differentiation are located in the discrete regions of homology between the c- and L-myc genes.
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