Silver nanoparticles were synthesized by reduction method using glucose as reducing agent for precursor AgNO3. This research was aimed at comparing the stability and performance of silver nanoparticles with stabilizer gelatin (Gelatin-AgNPs) and tween-20 (Tween-AgNPs) produced from the synthesis to the silver nanoparticles without stabilizer, and applying the Gelatin-AgNPs and Tween-AgNPs to detect heavy metal in water sample. The silver nanoparticles produced were characterized using UV-Vis spectrophotometer and Transmission Electron Microscopy (TEM). From measurement of UV-Vis spectrophotometer, the absorbance wavelength of silver nanoparticles (AgNPs) appeared in range 411 nm, Gelatin-AgNPs in 417 nm, and Tween-AgNPs in 420 nm. The identification using TEM showed the average size for each AgNPs, Gelatin-AgNPs, and Tween-AgNPs was 11.73, 9.68, and 17.54 nm, respectively. The result showed that Gelatin-AgNPs has better stability compared to Tween-AgNPs. The reaction of Gelatin-AgNPs and Tween-AgNPs with several ions showed color changes of Gelatin-AgNPs and Tween-AgNPs occurred only on addition to Hg2+ metal ions solution. Based on the experiment of Hg2+ metal ions determination this method has limit of detection of 0.45 mg/L for Gelatin-AgNPs and 0.13 mg/L for Tween-AgNPs.
Background: Some Malaysia and Indonesia people believed that root and seed shell of Archidendron bubalinum can treat diabetes. However, seed shell of Archidendron bubalinum has not yet to be scientifically proven and confirmed their ability to treat diabetes. The study of the potential of this seed shell was also scarcely available. Objective: The recent work was aimed to investigate the phytochemical screening of methanol extracts of seed shells of Archidendron bubalinum and to evaluate their chemical compositions and antidiabetic activities. Material and Methods: The methods of phytochemical screening were including alkaloids, flavonoids, tannins, polyphenols, saponins, and terpenoids. Their chemical compositions were determined by Liquid Chromatography-Mass Spectrometry (LC-MS) and antidiabetic activities were performed by α-glucosidase inhibitory method. Results: The phytochemical screening showed that methanol extracts of seed shells of Archidendron bubalinum contain flavonoids, tannins, polyphenols, and terpenoids. This extracts exhibited antidiabetic activity with IC 50 7.77 µg/mL. This result was supported by LC-MS analysis which showed the presence of phlorizin and astilbin, in which these compounds had high inhibitory activity against α-glucosidase or diabetes. Conclusion: LC-MS analysis revealed the presence of polyphenol compounds namely phlorizin and astilbin in which had high α-glucosidase inhibitory activity, might largely contribute in the antidiabetic activity.
Abstract:The Pometia pinnata fruit flesh from Papua, Indonesia is widely used in traditional medicine has been extracted. The extraction has used the solvent of methanol, ethyl acetate, and nhexane. The yield of extraction in methanol, ethyl acetate, and n-hexane were 21.65%, 2.92% and 0.91%. Qualitative analysis of phytochemical constituents in methanol were tannins, phenolic and steroid, more complete contents of secondary metabolite, compared to those of n-hexane and ethyl acetate extracts. Quantitative analysis for total phenolic compound was 393.38±0.28 mg gallic acid equivalent (GAE.g -1 ).
This study focused on identifying the types of compounds contained in the ethyl acetate extract of seed shell of Archidendron bubalinum (Jack) I.C. Nielsen (AbJICN ) from Lampung Indonesia using Liquid Chromatograph Mass Spectrophotometer (LC-MS) and determining the potential of anti-uric acid in the identified compounds. The experiment consists of four stages, which are 1) extraction the fruit seed shell of AbJICN using ethyl acetate, 2) phytochemical test, 3) determination of chemical structure using LC-MS and 4) anti uric acid test in vitro by Tri Bromo Hidroksibenzoid Acid (TBHBA) method. The result of phytochemical test found that ethyl acetate extract in fruit seed shell of AbJICN contains compound of flavanoid and terpenoid group. This founding was also supported by the characterization of chemical compounds using LC-MS which indicated the presence of candidates allopurinol and 2 4 7-Trinitrofluorenone compounds. The decreased uric acid by ethyl acetate extract of fruit seed shell was 1.25 mg/dL half times compared to standard allopurinol 2,43 mg/dL. It can be concluded that allopurinol compounds contained in the ethyl acetate extract of seedshell of Archidendron bubalinum (Jack ) I.C. Nielsen potentially became an active anti uric acid substance.
Keywords-Seed shell of Archidendron bubalinum (Jack) I.C. Nielsen (AbJICN; Liquid Chromatograph Mass Spectrophotometer (LC-MS); allopurinol; 2 4 7-Trinitrofluorenone; anti uric acid
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