CLA has been studied for its beneficial effects on health. However, the possibility of adverse effects, such as increased oxidative stress, must also be considered. The present work aims to assess the effect of CLA supplementation on the process of lipid autoxidation, both in the presence and in absence of an antioxidant. The investigation consisted in a biological assay with 60 rats divided into six groups: C (control), CE (control + vitamin E), AE (AdvantEdgeCLA), AEE (AdvantEdgeCLA + Vitamin E), CO (CLA One) and COE (CLA One)+ vitamin E). The CLA amount was 2% of feed consumption. Animals were supplemented for 42 days. As indicators of lipid autoxidation, peroxide (IP), malondialdehyde (MDA), 8-iso-PGF2(alpha) isoprostane and catalase were determined. Hepatic IP results indicated that CLA increased oxidation: values for CLA-supplemented groups, particularly group CO (84.38 +/- 10.97 mequiv/kg), were higher than those of the control group (54.75 +/- 9.70 mequiv/kg). In contrast, serum MDA results showed that CLA reduces oxidation both for group AE (1.8 +/- 0.67 mg of MDA/l) and for group CO (2.43 +/- 0.61 mg of MDA/l) as compared to the control group (3.85 +/- 0.24 mg of MDA/l). Serum catalase indicated a reduction of oxidation: groups AE and CO displayed 4734.23 +/- 1078.93 kU/l and 5916.06 +/- 2490.71 kU/l, respectively. These values are significantly lower than those of the control group. An increase in 8-iso-PGF2(alpha) in urine was observed, particularly in group AE (95.13 +/- 20.26 pg/ml) as compared to the control group (69.46 +/- 16.65 pg/ml). It was concluded that the influence of CLA on lipid autoxidation is dependent on supplement type, supplement dosage and chosen indicator, including its tissue and determination methodology.
O ácido linoléico conjugado, um conjunto de isômeros geométricos e de posição do ácido linoléico, vem sendo muito estudado devido ao seu efeito sobre a composição corporal, promovendo redução da massa gorda. O objetivo deste trabalho foi avaliar o efeito da suplementação com ácido linoléico conjugado sobre a composição corporal de ratos Wistar saudáveis em crescimento. Um total de 40 ratos, divididos em quatro grupos, foram suplementados diariamente durante três semanas com AdvantEdge® ácido linoléico conjugado (EAS TM) nas concentrações 1,0%, 2,0% e 4,0% sobre o consumo diário de dieta, constituindo, respectivamente, os grupos AE1, AE2 e AE4, e com ácido linoléico na concentração de 2% sobre o consumo diário de dieta, constituindo o grupo-controle. Os animais foram suplementados por meio de entubação orogástrica. Para a avaliação da composição corporal centesimal foi removido o conteúdo intestinal para obtenção da carcaça vazia. Em seguida, a carcaça foi congelada em nitrogênio líquido, fatiada, liofilizada, moída e armazenada a -25°C, até o momento das determinações de umidade, cinzas, proteína bruta e gordura. O grupo AE1 apresentou maior consumo de dieta e ganho de peso, mas não diferiu quanto à eficiência alimentar dos demais grupos (p< 0,05). Em relação à composição corporal, constatou-se redução dos teores de gordura corporal dos grupos AE2 (11,2%) e AE4 (11,6%), quando comparados ao teor do grupo-controle (13,9%). A suplementação com ácido linoléico conjugado nas concentrações de 2,0% e 4,0% sobre o consumo diário de dieta promoveu redução de gordura corporal de 18,0% nos ratos Wistar.
Rev. Nutr., Campinas, 22 (5) A B S T R A C TSupplementation with conjugated linoleic acid may reduce fat body mass and increase lean body mass in various species. Some studies have demonstrated that conjugated linoleic acid reduces body fat, in part, by inhibiting the activity of lipoprotein lipase in adipocytes. The objective of this work was to study the effect of conjugated linoleic acid supplementation on lipoprotein lipase activity in 3T3-L1 adipocyte culture. 3T3-L1 adipocytes received linoleic acid (group C) or conjugated linoleic acid (group AE, supplemented with AdvantEdge ® CLA, and group CO, supplemented with CLA One ® ) in concentrations of 1 mmol/L. Heparin-releasable lipoprotein lipase activity was analyzed by means of a 3T3-L1 adipocyte culture. After 7 days, heparin-releasable lipoprotein lipase activity was lower in the groups AE and CO supplemented with conjugated linoleic acid. These results suggest that one of the mechanisms by which CLA is capable of reducing body fat is by reducing lipoprotein lipase activity.
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