Effect of conjugated linoleic acid supplementation on lipoprotein lipase activity in 3T3-L1 adipocyte culture

Abstract

Rev. Nutr., Campinas, 22 (5) A B S T R A C TSupplementation with conjugated linoleic acid may reduce fat body mass and increase lean body mass in various species. Some studies have demonstrated that conjugated linoleic acid reduces body fat, in part, by inhibiting the activity of lipoprotein lipase in adipocytes. The objective of this work was to study the effect of conjugated linoleic acid supplementation on lipoprotein lipase activity in 3T3-L1 adipocyte culture. 3T3-L1 adipocytes received linoleic acid (group C) or conjugated linoleic acid (group AE, supplemented with AdvantEdge ® CLA, and group CO, supplemented with CLA One ® ) in concentrations of 1 mmol/L. Heparin-releasable lipoprotein lipase activity was analyzed by means of a 3T3-L1 adipocyte culture. After 7 days, heparin-releasable lipoprotein lipase activity was lower in the groups AE and CO supplemented with conjugated linoleic acid. These results suggest that one of the mechanisms by which CLA is capable of reducing body fat is by reducing lipoprotein lipase activity.

Eicosapentaenoic and docosahexaenoic acid supplementation during early gestation modified relative abundance on placenta and fetal liver tissue mRNA and concentration pattern of fatty acids in fetal liver and fetal central nervous system of sheep

“…In humans, LPL is a candidate gene for obesity, based on the function of the protein encoded by this gene to induce absorption of fatty acids across the cell walls of tissues. When there was supplementation of conjugated linoleic acid, there was confirmation of the capacity of this compound to reduce LPL activity, indicating that the inhibition of LPL activity seems to be a mechanism underlying body fat reduction [31].…”

The effects of conjugated linoleic acid isomers on the morphological changes in adipose tissue and adipogenic genes expressions on primary adipose tissue

“…Study by Zhou et al (2007) demonstrated LPL mRNA in cultures treated with CLA remained unchanged. In contrast, Botelho et al (2009) found that the LPL activity was reduced. Similar changes were also observed in the in vivo studies when the mice were treated with CLA (Park et al, 1997).…”

“…Adipose tissue acquired its ability to respond to insulin, and expressed fat metabolism-related genes such as lipoprotein lipase (LPL), adiposin and glucose transporter 4 (GLUT4) during their differentiation into mature adipocytes (Botelho et al, 2009). Study by Zhou et al (2007) demonstrated LPL mRNA in cultures treated with CLA remained unchanged.…”

Conjugated Linoleic Acid Combined with Physical Activity Reduces Body Fat Accumulation But Does Not Modify Lean Body Mass in Male and Female Wistar Rats

“…4 The main mechanism of action of CLA is the reduction in lipogenesis, which has been the subject of extensive research. [5][6][7][8] The antilipogenic activity of CLA was initially verified through an experiment that demonstrated a significant reduction in lipogenesis due to the inhibition of lipoprotein lipase located on the surface of adipocytes, which would in fact limit the incorporation of fatty acids into the cell. 5 An increase in the carnitine palmitoyltransferase activity in the mitochondria of muscle cells was also verified.…”