In this paper, the efficient quenching effect of deoxyguanosine-5'-phosphate (dGMP) on anodic electrochemiluminescence (ECL) of the CdTe/ZnS quantum dots (QDs) is reported for the first time. This ECL quenching was found to be specific for free dGMP and not observed for dGMP residues in different DNA structures. The unique dGMP-based QDs ECL quenching was then utilized to develop a versatile biosensing strategy to determine various protein-DNA interactions with the assistance of exonuclease, Exo I, to hydrolyze DNA and liberate dGMP. Taking single-stranded DNA binding protein (SSB) and thrombin as examples, two novel detection modes have been developed based on dGMP-QDs ECL strategy. The first method used hairpin probes and SSB-promoted probe cleavage by Exo I for facile signal-off detection of SSB, with a wide linear range of 1-200 nM and a low detection limit of 0.1 nM. The second method exploited aptamer-thrombin binding to protect probes against Exo I degradation for sensitive signal-on detection of thrombin, giving a linear response over a range of 1-150 nM and a detection limit as low as 0.1 nM. Both methods were homogeneous and label-free without QDs or DNA modification. Therefore, this dGMP-specific QDs ECL quenching presents a promising detection mechanism suitable for probing various protein-nucleic acid interactions.
Tris(2,3-dibromopropyl) isocyanurate (TBC) is a heterocyclic hexabrominated flame retardant, which cannot be degraded even over a long time and may be a potential environmental pollutant. In this paper, TBC is for the first time as far as we know determined by silver nitrate-enhanced electrochemiluminescence (ECL) using a gold nanoparticles (AuNPs)-modified gold electrode. In our experiments, TBC was found to have the characteristics of increasing the ECL intensity of Ru(bpy)(3)(2+), and the ECL signal was proportional to the concentration of TBC. Based on this, we have successfully developed a novel, fast and sensitive method for the analysis of TBC. The main influencing factors including the volume ratio of acetonitrile and water, and the concentration of Ru(bpy)(3)(2+) were investigated in detail. Compared with using a bare gold electrode in MeCN without AgNO(3), the limit of detection is remarkably lowered by 20 times and the linear range is expanded by 5 times by using the AuNPs-modified gold electrode and AgNO(3). Under the optimal conditions, a limit of detection of 5.0 × 10(-8) M (S/N = 3) is achieved with a linear range of 1.0 × 10(-7) to 5.0 × 10(-5) M. The mechanism of the ECL enhancement of the system is also investigated.
The present study experimentally and numerically investigates the feasibility of applying multiple dielectric barrier discharge (multi-DBD) plasma actuators to reduce wind loads and modify local flow field on the roofs of low-rise buildings. Four arrangements of multi-DBD plasma actuators are placed on the roofs and induce four directions of wall jets. The results show that mean and peak negative pressure coefficients are negative no matter the plasma actuation is on or off. These wall jets decrease the absolute values of these pressure coefficients in different roof regions and succeed in modifying the local flow around the roof. The wall jet blowing from the trailing edge to the leading edge has the best effect on wind loads, since the absolute values of mean and peak negative pressure coefficients are respectively reduced by about 20% and 12% near the leading edge. The different variations of wind loads and the local flow for all arrangements are due to the compact of the directionality of the wall jets. The wall jet produces a strong suction region at the beginning of the wall jet, which leads to an obvious enhancement of wind loads in this region. Meanwhile, the wall jet induces a strong pressure region at the back of the wall jet, resulting in an apparent decline of wind loads.
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