Molybdenum oxide nanomaterials have become a popular nanomaterial while its catalysis behavior especially the bio‐catalysis property is little understood, even though molybdenum has been employed as a key cofactor in several important enzymes in biological system. On the basis of these, the Fenton and peroxidase‐like property of molybdenum oxide quantum dots (MoOx QDs) with mixed valence state was carefully investigated using tetramethylbenzidine (TMB) and methyl orange (MO) as the substrates. The results showed that MoOx QDs not only serve as a Fenton reagent, degrading MO to colorless products, but also exhibit intrinsic peroxidase‐mimetic activity, oxidizing TMB to the oxidized state oxTMB. The catalysis mechanism of MoOx QDs is that MoOx QDs can facilitate the decomposition of H2O2 and a large amount of radicals was generated, leading to MO decomposing and TMB oxidizing subsequently. Inspired by the findings, a visible method was developed for sensitive and selective detection of H2O2 and glucose, and the limit of detection for H2O2 and glucose is 7.75 μM and 8.95 μM (3σ/K), respectively. Moreover, glucose content in human serum samples was successfully determined by the proposed visible method, and the results are consistent well with those obtained by handheld glucose meter, suggesting a high accuracy of the present method.
Tuberculosis is one of the main infectious diseases threatening public health, and the development of simple, rapid, and cost‐saving methods for tuberculosis diagnosis is of profound importance for tuberculosis prevention and treatment. The bacterium Mycobacterium tuberculosis (MTB) is the pathogen that causes tuberculosis, and assaying for MTB is the only criterion for tuberculosis diagnosis. A new enzyme‐free method based on strand displacement amplification and magnetic beads was developed for simple, rapid, and cost‐saving MTB detection. Under optimum conditions, a good linear relationship could be observed between fluorescence and MTB specific DNA concentration ranging from 0.05 to 150 nM with a correlation coefficient of 0.993 (n = 8) and a detection limit of 47 pM (3σ/K). The present method also distinguished a one base mismatch from MTB specific DNA, showing great promise for MTB genome single base polymorphism analysis. MTB specific DNA content in polymerase chain reaction samples was successfully detected using the new method, and recoveries were 97.8–100.8%, indicating that the present method had high accuracy and shows good potential for the early diagnosis of tuberculosis.
The density functional theory (DFT) is used to compute the ground-state geometries of naphthoquinone derivatives, and lowest singlet excited-state geometries of them have been investigated by the singles configuration interaction (CIS) method. The absorption and emission spectra are calculated by time-dependent DFT (TDDFT) on the basis of the ground- and excited-state geometries, respectively. Our calculations are in good agreement with the available experimental results. The calculated results show that with the introduction of hydroxyl the red-shift was found in the absorption and emission, and the range of spectra reach the visible region. Furthermore, in the absorptions electron transition type was identified from the point-view of molecular orbitals. Study of the effect of hydroxyl and site on spectra can provide the helpful information on further designing molecular devices.
Due to the emergency plan format information characteristics is stronger and more easily to gain the complete content description, so this paper puts forward the recommendation algorithm of emergency plan based on content filtering. This paper proposes the characteristics model of emergency plan and the user's interest model and designs the flexible recommendation interface for user interactive, so we can get the recommend results.
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