Rapid and sensitive detection of Mycobacterium tuberculosis based on strand displacement amplification and magnetic beads

Abstract

Tuberculosis is one of the main infectious diseases threatening public health, and the development of simple, rapid, and cost‐saving methods for tuberculosis diagnosis is of profound importance for tuberculosis prevention and treatment. The bacterium Mycobacterium tuberculosis (MTB) is the pathogen that causes tuberculosis, and assaying for MTB is the only criterion for tuberculosis diagnosis. A new enzyme‐free method based on strand displacement amplification and magnetic beads was developed for simple, rapid, and cost‐saving MTB detection. Under optimum conditions, a good linear relationship could be observed between fluorescence and MTB specific DNA concentration ranging from 0.05 to 150 nM with a correlation coefficient of 0.993 (n = 8) and a detection limit of 47 pM (3σ/K). The present method also distinguished a one base mismatch from MTB specific DNA, showing great promise for MTB genome single base polymorphism analysis. MTB specific DNA content in polymerase chain reaction samples was successfully detected using the new method, and recoveries were 97.8–100.8%, indicating that the present method had high accuracy and shows good potential for the early diagnosis of tuberculosis.

Advances in magnetic nanoparticles for the separation of foodborne pathogens: Recognition, separation strategy, and application

“…The molecular detection method based on nucleic acid is still a prime method for the detection of foodborne pathogens, including the conventional polymerase chain reaction (PCR) (Y. Luo et al., 2017), and novel isothermal amplification methods, such as Loop‐mediated isothermal amplification (J. Feng et al., 2018), rolling circle amplification (Y. Wang, Wang, et al., 2022), hybridization chain reaction (G. Yang et al., 2020; B. Yu, Li, et al., 2018), strand displacement amplification (Z. J. Chu et al., 2021), and recombinase aided amplification (Fang et al., 2021). Nucleic acid‐based methods have advantages in specificity and sensitivity, but low‐level target pathogens still need to be enriched to detect, and the performance of most nucleic acid‐based analytical methods is affected by the purity of the nucleic acid (Y. Chen et al., 2020).…”

A carbon-black-embedded poly(dimethylsiloxane)-paper hybrid device for energy-efficient nucleic-acid amplification in point-of-care testing

“…5 An infrared (IR) tungsten lamp requires 50 W. 6 By contrast, isothermal amplication methods can amplify nucleic acids at a constant temperature. Methods such as transcription-mediated amplication, 7 loop-mediated amplication (LAMP), 8 helicase-dependent amplication, 9 and stranddisplacement amplication 10 have been proven to be effective in amplifying the target genetic material. Additionally, isothermal amplications are well-suited for POCT, because they can be performed under a constant temperature, thus simplifying the temperature-control mechanism.…”