Background:Ginseng is Chinese traditional herbal medicine, and the ginsenoside Rg3 is the main bioactive ingredient for the anti-tumor effect. However, there is no study on pharmacokinetics (PKs) of ginsenoside Rg3 and its main metabolite after oral ginsenoside Rg3 in tumor-bearing plasma. The aim of this study was to investigate the PK profiles of ginsenoside Rg3 and ginsenoside Rh2 after oral administration of pure ginsenoside Rg3 were administered, and compare the difference of the PK profiles between normal and Walker 256 tumor-bearing rats.Materials and Methods:The concentrations of two ginsenosides in plasma were determined by using a simple and rapid high-performance liquid chromatography. All the rats were divided randomly into two groups (Walker 256 tumor-bearing and normal groups). Each group received oral administration of 50 mg/kg ginsenoside Rg3.Results:The results showed that ginsenoside Rh2, possibly as a glycosylation hydrolysis product of ginsenoside Rg3, were found in plasma after oral administration of ginsenoside Rg3 to rats. Ginsenoside Rg3 had shown better absorption than ginsenoside Rh2, whether the oral administration of ginsenoside Rg3, normal rats showed better absorption than tumor-bearing rats.Discussion and Conclusion:The PKs properties of the ginsenoside Rg3 and ginsenoside Rh2 differed between tumor-bearing rats and normal rats, including area under the plasma level/time curve and concentration maximum (P < 0.05).SUMMARY
Ginsenoside Rh2 was found in plasma after oral administration of ginsenoside Rg3 to ratsHPLC could be used to determine simultaneously, the concentration of ginsenoside Rg3 and ginsenoside Rh2 in rat plasma after oral administration of ginsenoside Rg3Normal rats showed better absorption than tumor-bearing rats after oral administration of ginsenoside Rg3.0.
A sensitive and selective analytical method based on high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been developed for the simultaneous determination of rimonabant and orlistat in weight-loss functional foods. After the foods were extracted under accelerated solvent extraction, the HPLC separation was performed on a Waters Atlantis T3 column (150 mm x 2.1 mm, 3 microm) with a linear gradient elution program of methanol and 10 mmol/L ammonium acetate as the mobile phase. Electrospray ionization was applied and operated in the positive ion mode. The results showed that the limits of detection for the rimonabant and orlistat were 0.5 mg/kg. The calibration curves showed good linearities for rimonabant and orlistat in the range of 0.5 - 100 microg/L, and the correlative coefficients were 0.998 9 and 0.999 4, respectively. The mean recoveries at the 3 spiked levels (2, 5, 10 mg/kg) were 80.5% - 102.1% with the intra-day precision less than 6% and the inter-day precision less than 8%. The work studied the mass spectrum characterization of the 2 drugs and speculated on the fragmentation pathways. The method is sensitive, reproducible and adapts to the determination of rimonabant and orlistat in different weight-loss functional foods.
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