Ellagic acid (EA) is able to inhibit the growth of several cancer cells; however, its effect on human ovarian carcinoma cells has not yet been investigated. Ovarian carcinoma ES-2 and PA-1 cells were treated with EA (10~100 μM) and assessed for viability, cell cycle, apoptosis, anoikis, autophagy, and chemosensitivity to doxorubicin and their molecular mechanisms. EA inhibited cell proliferation in a dose- and time-dependent manner by arresting both cell lines at the G1 phase of the cell cycle, which were from elevating p53 and Cip1/p21 and decreasing cyclin D1 and E levels. EA also induced caspase-3-mediated apoptosis by increasing the Bax : Bcl-2 ratio and restored anoikis in both cell lines. The enhancement of apoptosis and/or inhibition of autophagy in these cells by EA assisted the chemotherapy efficacy. The results indicated that EA is a potential novel chemoprevention and treatment assistant agent for human ovarian carcinoma.

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We hypothesized that induction of nitric oxide synthase and cyclo-oxygenase-2 by bacterial products in intra-amniotic infection could increase the production of proinflammatory nitric oxide and prostaglandin E2 (PGE2) and cause preterm labor. Thus, we sought to determine amniotic fluid levels of nitric oxide metabolites (NOx) and PGE2 in preterm labor patients with and without intra-amniotic infection. Amniotic fluid from 13 preterm labor patients with intra-amniotic infection and 24 without intra-amniotic infection were studied. Intra-amniotic infection was defined as the presence of a positive amniotic fluid culture. Amniotic fluid was tested for NOx, PGE2, glucose, leukocyte counts, Gram stains, creatinine, pH, and specific gravity. NOx was determined using Griess reagent after reduction of nitrate to nitrite with aspergillus nitrate reductase. PGE2 was measured by an enzyme-linked immunoassay. Both amniotic fluid NOx and PGE2 were normalized by amniotic fluid creatinine. We found that amniotic fluid concentrations of NOx and PGE2 were significantly higher in preterm labor patients with intra-amniotic infection compared to those without intraamniotic infection (NOx: median 1.8 micromol/mg creatinine, range 0.7 to 6.8 vs. 1.3 micromol/mg creatinine, range 0.9 to 2.1, p=0.03; PGE2: median 33.5 ng/mg creatinine, range 0.0 to 1048.6 vs. 0.0 ng/mg creatinine, range 0.0 to 33.6, p=0.004). In addition, amniotic fluid NOx and PGE2 were positively correlated (r=0.343, p=0.0398). We conclude that there may be an interaction between the nitric oxide and prostaglandin pathways in intraamniotic infection. Increased production of amniotic fluid pro-inflammatory nitric oxide and PGE2 may play an important role in the pathogenesis of preterm labor in patients with intra-amniotic infection.

NGC 4945 is a Seyfert 2 galaxy at a distance of 3.82 Mpc. Its relative proximity has permitted a detailed Submillimeter Array study of the circumnuclear molecular gas in a galaxy exhibiting an active galactic nucleus (AGN). Based on an analysis of the high-resolution velocity field of the central region (20 × 20 , 1 = 19 pc), we demonstrate that the S-shaped structure of the isovelocity contours is well reproduced by the numerical results of a two-dimensional hydrodynamical simulation. In particular, the velocity structure is represented by the bending produced by a shock along the spiral density waves, which are excited at the outer-inner Lindblad resonance (OILR) by a fast rotating bar. The simulated density map reveals a pair of tightly wound spirals in the center which pass through most of the ring-like (claimed to be a circumnuclear starburst ring by other authors) high-intensity region in the observations as well as intersect several Paα emission line knots located outside the ring-like region. The calculated mass inflow rate at a scale of 50 pc is about three times the inferred mass accretion rate of the AGNs of NGC 4945. We find that self-gravity of the gas is important and should be included in our model for NGC 4945. The model is compared with the gas orbit model discussed in Lim et al., and it is shown that the hydrodynamic model provides a better match to the observed position-velocity diagram and, hence, provides a more reliable prediction of the OILR position.

NGC 6782 is an early-type barred spiral galaxy exhibiting a rich and complex morphology with multiple ring patterns. To provide a physical understanding of its structure and kinematical properties, two-dimensional hydrodynamical simulations have been carried out. Numerical calculations reveal that the striking features in NGC 6782 can be reproduced provided that the gas flow is governed by the gravitational potential associated with a slowly rotating strong bar. In particular, the response of the gaseous disk to the bar potential leads to the excitation of spiral density waves at the inner Lindblad resonance giving rise to the appearance of a nearly circular nuclear ring with a pair of dust lanes. For a sufficiently strong bar potential, the inner 4:1 spiral density waves are also excited. The interaction of the higher harmonic waves with the waves excited at the inner Lindblad resonance and confined by the outer Lindblad resonance results in the observed diamond-shaped (or pointy oval) inner ring structure. The overall gas morphology and kinematical features are both well reproduced by the model provided that the pattern speed of the bar is $\sim 25$ km s$^{-1}$ kpc$^{-1}$.Comment: 35 pages, 14 figure