The antigenic polymorphism of HIV-1 is a major obstacle in developing an effective vaccine. Accordingly, we screened random peptide libraries (RPLs) displayed on phage with antibodies from HIV-infected individuals and identified an array of HIV-specific epitopes that behave as antigenic mimics of conformational epitopes of gp120 and gp41 proteins. We report that the selected epitopes are shared by a collection of HIV-1 isolates of clades A-F. The phage-borne epitopes are immunogenic in rhesus macaques, where they elicit envelope-specific antibody responses. Upon intravenous challenge with 60 MID50 of pathogenic SHIV-89.6PD, all monkeys became infected; however, in contrast to the naive and mock-immunized monkeys, four of five mimotope-immunized monkeys experienced lower levels of peak viremia, followed by viral set points of undetectable or transient levels of viremia and a mild decline of CD4+ T cells, and were protected from progression to AIDS-like illness. These results provide a new approach to the design of broadly protective HIV-1 vaccines.
Admission Rorschach variables were compared to 2-year reevaluation responses of adolescents (n = 50) in residential treatment using Weiner and Exner's (1991) 27 structural variables. Fifty adolescents who had previously failed to improve in outpatient treatment and multiple hospitalizations were subsequently placed in residential treatment. Following 2 years of residential treatment, they showed positive personality changes as indicated by a reduction in Rorschach indices of impaired functioning. Positive treatment changes in interpersonal awareness were discussed utilizing a social identification index formulated for this population. Results indicate the utility of the Rorschach test-retest method of assessing treatment outcome.
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