KeyWords Column liquid chromatography Echinacea purpurea Phenolic compounds Alkamides SummaryThe two major classes of compound (hydrophilic and lipophTITc) From Echinacea purpureo L. Moench, responsible for the TmmunestTmulatTng effect of the plant, have usually been determTned by two different methods. This paper describes simultaneous analysts by high-performanco liquid chromatography (HPLC) of hydrophilic compounds and lipophilic compounds (alkamides) from pharmaceutical preparations of Echinacea purpurea root.A linear binary gradient, at a flow of 1 m/rain 1 starting wth 1:9 acotonitrile water (both containing O. 1% orthophosphor~c acid) and ending with 8:2 acetonitrile water (both contain ing 0.1% orthophosphor~c acid) aflerSO min, was optimized for separation of the compounds.Hydrophilic compounds were separated in the first 25 min and the lipophilic compounds between 25 and 50 rain. The compounds were identified on the basis of correlation of retention times and spectral data. The external standard method was used For quantitatve analysts.

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Conclusion: Our data show that AZA monotherapy is safe and effective in controlling disease both in upfront and relapsed patients in order to proceed to HSCT.

Because melatonin has strong antioxidant activity and wine is an alcoholic beverage of economic relevance, in the present work, the impact of some variable parameters that may occur in the winemaking process on the concentrations of melatonin and its precursors in Romanian wines was studied. Therefore, a sensitive and selective high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the simultaneous analysis of melatonin, serotonin, and l -tryptophan, and some method performance parameters including selectivity, detection limit, precision (by comparing with an alternative HPLC-FL method), accuracy, and robustness were validated. These determinations are significant and the final amounts of analytes are dependent on the microorganisms involved in the winemaking process, the grape variety, geographic regions of vineyards, and aging of wines. In the future, the method may be useful to increase the melatonin content and the antioxidant activity in wines by improved steps in the winemaking process, especially based on application of selected yeasts and improved fermentation conditions.

The international standard protocol for acute lymphoblastic leukaemia (ALL)