To date, mutation of conserved N-terminal residues in endogenous histone genes has only been performed in budding yeast, which lacks the Clr4/Suvar39 histone methyltransferase and Swi6/HP1. We demonstrate the importance of conserved residues within the histone H3 N terminus for the maintenance of centromeric heterochromatin in fission yeast. In sharp contrast, mutation of two conserved lysines within the histone H4 tail has no impact on the integrity of centromeric heterochromatin. Our data highlight the striking divergence between the histone tail requirements for the fission yeast and budding yeast silencing pathways.
This article discusses the results of a survey of SMIS members. Members were asked to respond to questions regarding demographics, their satisfaction with SMIS services, and the importance of eighteen management issues which MIS management might address. The article presents percentage results of individual questions and interprets the results of those questions. No attempt is made to use any bivariate statistics in this article. All questions asked on the survey are presented in this article.
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