The microbial fermentation in the stomachs of two monkeys, Colobus polykomos, collected in Kenya, was studied. The gas accumulated within the stomach contained H2 but no CH,. Volatile fatty acid concentrations were high, but accumulated acid prevented determination of the fermentation rate in untreated, incubated stomach contents. Upon addition of bicarbonate, a very rapid rate could be demonstrated. Some D-and L-lactate were in the stomach contents. Starchy seeds or fruits rather than leaves appeared to have been consumed. Microscopically, the most prominent microorganisms seen were large, very refringent cocci, possibly Sarcina ventriculi, and various smaller cocci and rods. The 28 cultured strains of bacteria included 14 Staphylococcus, 2 Streptococcus, 10 Propionibacterium, and 2 Peptostreptococcus. The culture count constituted 10 to 20% of the direct count. No protozoa or cellulolytic bacteria were found.
Eight coagulase-negative, enterotoxigenic strains of cocci and one weakly coagulase-positive strain isolated from a number of different sources, including cases of food poisoning incidents, were evaluated for their relationship to Staphylococcus aureus on the basis of deoxyribonucleic acid (DNA) buoyant
The minimal water activity (a,) for growth was correlated with enterotoxin A formation by two strains of Staphylococcus aureus in a salt mixture broth. Within 7 days at 300C both strains grew and formed enterotoxin A minimally between a. 0.864 and 0.867, but at 25°C, the minimal a. for both activities was increased to between 0.870 and 0.887 after a 2-week incubation.
The microbial fermentation in the stomachs of two monkeys,
Colobus polykomos
, collected in Kenya, was studied. The gas accumulated within the stomach contained H
2
but no CH
4
. Volatile fatty acid concentrations were high, but accumulated acid prevented determination of the fermentation rate in untreated, incubated stomach contents. Upon addition of bicarbonate, a very rapid rate could be demonstrated. Some D- and L-lactate were in the stomach contents. Starchy seeds or fruits rather than leaves appeared to have been consumed. Microscopically, the most prominent microorganisms seen were large, very refringent cocci, possibly
Sarcina ventriculi
, and various smaller cocci and rods. The 28 cultured strains of bacteria included 14
Staphylococcus
, 2
Streptococcus
, 10
Propionibacterium
, and 2
Peptostreptococcus
. The culture count constituted 10 to 20% of the direct count. No protozoa or cellulolytic bacteria were found.
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