The widespread occurrence of Palmer amaranth resistant to acetolactate synthase inhibitors and/or glyphosate led to the increased use of protoporphyrinogen oxidase (PPO)-inhibiting herbicides. This research aimed to: (1) evaluate the efficacy of foliar-applied fomesafen to Palmer amaranth, (2) evaluate cross-resistance to foliar PPO inhibitors and efficacy of foliar herbicides with different mechanisms of action, (3) survey the occurrence of the PPO Gly-210 deletion mutation among PPO inhibitor–resistant Palmer amaranth, (4) identify other PPO target-site mutations in resistant individuals, and (5) determine the resistance level in resistant accessions with or without the PPO Gly-210 deletion. Seedlings were sprayed with fomesafen (263 gaiha−1), dicamba (280 gaiha−1), glyphosate (870 gaiha−1), glufosinate (549 g ai ha−1), and trifloxysulfuron (7.84 gaiha−1). Selected fomesafen-resistant accessions were sprayed with other foliar-applied PPO herbicides. Mortality and injury were evaluated 21 d after treatment (DAT). The PPX2L gene of resistant and susceptible plants from a selected accession was sequenced. The majority (70%) of samples from putative PPO-resistant populations in 2015 were confirmed resistant to foliar-applied fomesafen. The efficacy of other foliar PPO herbicides on fomesafen-resistant accessions was saflufenacil>acifluorfen=flumioxazin>carfentrazone=lactofen>pyraflufen-ethyl>fomesafen>fluthiacet-methyl. With small seedlings, cross-resistance occurred with all foliar-applied PPO herbicides except saflufenacil (i.e., 25% with acifluorfen, 42% with flumioxazin). Thirty-two percent of PPO-resistant accessions were multiple resistant to glyphosate and trifloxysulfuron. Resistance to PPO herbicides in Palmer amaranth occurred in at least 13 counties in Arkansas. Of 316 fomesafen survivors tested, 55% carried the PPO Gly-210 deletion reported previously in common waterhemp. The PPO gene (PPX2L) in one accession (15CRI-B), which did not encode the Gly-210 deletion, encoded an Arg-128-Gly substitution. The 50% growth reduction values for fomesafen in accessions with Gly-210 deletion were 8- to 15-fold higher than that of a susceptible population, and 3- to 10-fold higher in accessions without the Gly-210 deletion.

Palavras-chave: arroz-vermelho, arroz Clearfield ® , imidazolinonas, persistência no solo.ABSTRACT -The aim of this study was to evaluate the residual activity of the prepackage mix of the herbicide Only (imazethapyr+imazapic) in a Clearfield (CL) rice production system during one, two or three rice seasons. The study was carried out in the field at Centro Agropecuário da Palma/ Universidade Federal de Pelotas, Rio Grande do Sul, southern Brazil. Ryegrass (Lolium multiflorum) was planted after CL rice, cv. IRGA 422 CL. CL rice was considered as the main crop (summer) and ryegrass was planted following CL rice. The experimental design was a randomized complete block, with four replications. The treatments were arranged as a factorial: factor A, during the same season, compared herbicide residues during one, two or three years of CL rice and factor B, for herbicide rates. Herbicide (imazethapyr+imazapic) was applied at (0; 25+75; 37,5+112,5; 50+150) g ha -1 with surfactant Dash being added at 0.5% v/v. The results showed interaction

Rice is the staple food for about half of the world population. Rice grain yield and quality are affected by climatic changes. Arguably, rice cultivars’ genetic diversity is diminished from decades of breeding using narrow germplasm, requiring introgressions from other Oryza species, weedy or wild. Weedy rice has high genetic diversity, which is an essential resource for rice crop improvement. Here, we analyzed the phenotypic, physiological, and molecular profiles of two rice cultivars (IRGA 424 and SCS119 Rubi) and five weedy rice (WR), from five different Brazilian regions, in response to heat and drought stress. Drought and heat stress affected the phenotype and photosynthetic parameters in different ways in rice and WR genotypes. A WR from Northern Brazil yielded better under heat stress than the non-stressed check. Drought stress upregulated HSF7A while heat stress upregulated HSF2a. HSP74.8, HSP80.2, and HSP24.1 were upregulated in both conditions. Based on all evaluated traits, we hypothesized that in drought conditions increasing HSFA7 expression is related to tiller number and that increase WUE (water use efficiency) and HSFA2a expression are associated with yield. In heat conditions, Gs (stomatal conductance) and E’s increases may be related to plant height; tiller number is inversely associated with HSPs expression, and chlorophyll content and Ci (intercellular CO2 concentration) may be related to yield. Based on morphology, physiology, and gene regulation in heat and drought stress, we can discriminate genotypes that perform well under these stress conditions and utilize such genotypes as a source of genetic diversity for rice breeding.

We aimed with this study to evaluate the effects of residual activity in soil of formulated mixtures of the herbicides imazethapyr + imazapic and imazapyr + imazapic on ryegrass and on rice cultivars, IRGA 424 and BRS Querencia. Two experiments were conducted under greenhouse in randomized blocks design with four replications in split-plot and split-split-plot designs, respectively for the first (E1) and second (E2) experiments. For E1, main plots allocated herbicides (imazethapyr + imazapic and imazapyr + imazapic), and subplots the doses [(0, 0.5, 1.0, 1.5 and 2.0 fold the label dose of imazethapyr + imazapic (1 L•ha −1) and imazapyr + imazapic (140 g•ha −1) plus 0.5% of the adjuvant Dash ®)]; for E2, sub-subplots allocated rice cultivars (BRS 424 and IRGA Querência). Phytotoxicity, plant height and shoot dry weight (E1 and E2) and plant stand (E2) were evaluated. Results showed that the annual ryegrass and rice cultivars IRGA 424 and BRS Querencia are sensitive to imazethapyr + imazapic and imazapyr + imazapic, serving as bioindicators of its residual activity in soils following Clearfield ® rice. Imazapyr + imazapic, applied post-emergence in irrigated rice at doses of 1.0, 1.5 and 2.0× the label dose, present longer residual activity in soil compared to imazethapyr + imazapic for ryegrass and non-Clearfield ® rice.

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Palmer amaranth is one of the most difficult-to-control weeds in row crop systems and has evolved resistance to several herbicide sites of action (SOAs). A late-season weed-escape survey had been conducted earlier to determine the distribution of protoporphyrinogen oxidase–inhibitor resistant Palmer Amaranth in Arkansas. The objective of this study was to evaluate the susceptibility of Arkansas Palmer amaranth accessions to commonly used herbicide SOAs. The SOAs evaluated were group 2 + 9, 3, 4, 5, 10, 14, 15, and 27, and the representative herbicide from each group was imazethapyr + glyphosate (79 + 860 g ha−1), trifluralin (1,120 g ha−1), dicamba (280 and 560 g ha−1), atrazine (560 g ha−1), glufosinate (594 g ha−1), fomesafen (395 g ha−1), S-metolachlor (1,064 g ha−1), and tembotrione (92 g ha−1), respectively. Palmer amaranth mortality varied among accessions across SOAs. Averaged across accessions, the mortality rates, by treatment in order from lowest to highest, were as follows: glyphosate + imazethapyr (16%), tembotrione (51%), dicamba at 280 g ha−1 (51%), fomesafen (76%), dicamba at 560 g ha−1 (82%), atrazine (85%), trifluralin (87%), S-metolachlor (96%), and glufosinate (99.5%). This study provides evidence that Palmer amaranth accessions with low susceptibility to glyphosate + imazethapyr, fomesafen, and tembotrione are widespread throughout Arkansas. Of the remaining SOAs, most Palmer amaranth accessions were sensitive; however, within each herbicide SOA, except glufosinate, control of some accessions was less than expected and resistance is suspected.

Palavras-chave: arroz-vermelho, imidazolinonas, persistência no solo, Oryza sativa.ABSTRACT -The aim of this work was to evaluate the residual soil activity of the herbicide Only (imazethapyr+imazapic) to susceptible rice, cv. IRGA 417, after one or three years of Clearfield ® (CL) rice cultivation. Four experiments were conducted at the Universidade Federal de Pelotas, Capão do Leão, RS, southern Brazil. The experimental design was a randomized complete block, with four or eight replications, respectively, for the two field experiments (EC 1 and EC 3 ) or the two greenhouse experiments (CV 1 and CV 3 ). The treatments were arranged as a factorial with factor A, in the same season, compared residuals per year and factor B, compared herbicide residual rates applied to CL rice. The herbicide used was Only -imazethapyr (75 g L -1 ) + imazapic (25 g L -1 ), applied at 0; 100; 150 and 200 g ha -1 with surfactant Dash added at 0.5% v/v. The herbicide was applied when CL rice was at the 3-4 leaf stage. The variables measured were aboveground dry biomass, 1000-grain weight and rice yield for experiments EC 1 and EC 3, and plant height, aboveground dry biomass and root dry biomass for experiments CV 1 and CV 3 . For statistical analysis, the data were pooled together. The results showed that the CL rice system may restrict the cultivation of non-tolerant rice after CL rice. The herbicide Only at the recommended rate (100 g ha -1 ) stays in the soil long enough to affect the growth and yield of susceptible rice.

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