The purpose was to evaluate the effect of air-blowing duration of three different adhesive systems on immediate or thermal aged resin-dentin shear bond strength (SBS). Human dentin surfaces were bonded with: one-step (Bond Force, BF), two-step (FL-Bond II, FLB) and three-step (Scotch Bond Multi-Purpose, SBMP) adhesive systems. Bonded surfaces from each group were air-blown for 0, 5, or 10 s and cured. Composite cylinders were built on the treated surfaces and cured. Half of the specimens from each group were tested immediately and the other halves were tested after thermal aging. Statistical analysis showed significant decrease in SBS after thermal aging compared to immediate testing in all groups, except BF after 5 s air-blowing or FLB and SBMP with no air-blowing (p>0.05). The results suggested that 5 s air-blowing is necessary to obtain a stable SBS for BF. However, extended airblowing duration of FLB and SBMP decreased the SBS significantly after thermal aging.
Patients with amelogenesis imperfecta (AI) have defective enamel; therefore, bonded restorations of patients with AI have variable success rates. To distinguish which cases of AI may have good clinical outcomes with bonded materials, we evaluated etching characteristics and bond strength of enamel in mouse models, comparing wild-type (WT) with those having mutations in amelogenin (Amelx) and matrix metalloproteinase-20 (Mmp20), which mimic 2 forms of human AI. Etched enamel surfaces were compared for roughness by scanning electron microscopy (SEM) images. Bonding was compared through shear bond strength (SBS) studies with 2 different systems (etch-and-rinse and self-etch). Etched enamel surfaces of incisors from Amelx knock-out (AmelxKO) mice appeared randomly organized and non-uniform compared with WT. Etching of Mmp20KO surfaces left little enamel, and the etching pattern was indistinguishable from unetched surfaces. SBS results were significantly different when AmelxKO and Mmp20KO enamel surfaces were compared. A significant increase in SBS was measured for all samples when the self-etch system was compared with the etch-and-rinse system. We have developed a novel system for testing shear bond strength of mouse incisors with AI variants, and analysis of these data may have important clinical implications for the treatment of patients with AI.
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