A modular and efficient method for the synthesis of α-substituted 1,2-dihydroquinolines is described. Under mild metal-free conditions, readily available N-carbamoyl 1,2-dihydroquinolines undergo oxidative C-H alkynylation, alkenylation, and allylation with a range of potassium trifluoroborates using TEMPO oxoammonium salt as an oxidant.
A fast method to calculate the iron loss of switched reluctance motor (SRM) is proposed. The key of the method is that the flux density waveforms in different parts of the motor can be obtained quickly. First, the static flux density data varying with phase current and rotor position in various parts of SRM are obtained by finite element method (FEM). Then, the static flux density data are stored in 2D look-up tables in Matlab/Simulink to get the flux density waveforms by look-up table method, and the obtained flux density waveforms are verified by FEM results. Since the flux waveforms are nonsinusoidal and non-linear, an accurate and fast model for the iron loss prediction is presented, and different modified factors are applied to calculate the hysteresis loss according to the classification of flux density waveforms. Based on that, the calculation model is established in Matlab/Simulink, and iron losses of a three-phase 6/4 structure SRM under different turn-on and turn-off angles and load conditions are calculated and analysed. Finally, measured results are presented to verify the precision of the calculation model.
Fusarium graminearum is one of the causal agents of Fusarium head blight and produces the trichothecene mycotoxin, deoxynivalenol (DON). Thioredoxin reductases (TRRs) play critical roles in the recycling of oxidized thioredoxin. However, their functions are not well known in plant pathogenic fungi. In this study, we characterized a TRR orthologue FgTRR in F. graminearum. The FgTRR-GFP fusion protein localized to the cytoplasm. FgTRR gene deletion demonstrated that FgTRR is involved in hyphal growth, conidiation, sexual reproduction, DON production, and virulence. The ΔTRR mutants also exhibited a defect in pigmentation, the expression level of aurofusarin biosynthesis-related genes was significantly decreased in the FgTRR mutant. Furthermore, the ΔTRR mutants were more sensitive to oxidative stress and aggravated apoptosis-like cell death compared with the wild type strain. Taken together, these results indicate that FgTRR is important in development and pathogenicity in F. graminearum.
This study sets forth an equivalent electric circuit (EEC) model including core losses and phase interactions to obtain a more accurate steady and dynamic performance of the high speed switched reluctance motor (SRM) during the design, analysis, and control of the SRM drive system. The magnetic equivalent circuit (MEC) method is used to calculate the flux linkage and torque quickly and relative accurately. Based on the principle of transformation between the MEC and EEC, the EEC model is derived from the MEC model. To enhance the model precision, the dynamic core losses and phase interactions are analysed and added to the EEC model. The relationship between the phase current and virtual current corresponding to different parts is deduced. Then the simulation model of the whole SRM driver systems is established in MATLAB/Simulink to obtain the phase current and virtual current corresponding to a different portion. The experimental and simulated results of the static MEC model and the whole SRM drive system including the EEC model provide the conclusive evidence for validating their practicability.
Golgins are coiled-coil proteins that play prominent roles in maintaining the structure and function of the Golgi complex. However, the role of golgin proteins in phytopathogenic fungi remains poorly understood. In this study, we functionally characterized Fusarium graminearum golgin protein RUD3, a homolog of ScRUD3/GMAP-210 in Saccharomyces cerevisiae and mammalian cells. Cellular localization observation revealed that RUD3 is located in the cis-Golgi. Deletion of RUD3 caused defects in vegetative growth, ascospore discharge, deoxynivalenol (DON) production, and virulence. Moreover, the Δrud3 mutant showed reduced expression of tri genes and impairment of the formation of toxisomes, both of which play essential roles in DON biosynthesis. We further used GFP-tagged SNARE protein SEC22 (SEC22-GFP) as a tool to study the transport between endoplasmic reticulum (ER) and Golgi, and observed that SEC22-GFP was retained in the cis-Golgi in the Δrud3 mutant. RUD3 contains the coiled coil (CC), GRAB-associated 2 (GA2), GRIP-related Arf binding (GRAB), and GRAB-associated 1 (GA1) domains; which except for GA1, are indispensable for normal localization and function of RUD3, whereas only CC is essential for normal RUD3–RUD3 interaction. Together, these results demonstrate how the golgin protein RUD3 mediates retrograde trafficking in the ER-to-Golgi pathway and is necessary for growth, ascospore discharge, DON biosynthesis, and pathogenicity in F. graminearum. IMPORTANCE Fusarium head blight (FHB) caused by the fungal pathogen Fusarium graminearum is an economically important disease of wheat and other small grain cereal crops worldwide, with limited effective control strategies are available. A better understanding of the regulation mechanisms of F. graminearum development, DON biosynthesis and pathogenicity is therefore important for development of effective control management of this disease. Golgins are attached via their extreme carboxy terminus to the Golgi membrane and involved in vesicle trafficking and organelle maintenance in eukaryotic cells. In this study, we systematically characterized a highly conserved Golgin protein RUD3, and found that it is required for vegetative growth, ascospore discharge, DON production, and pathogenicity in F. graminearum. Our findings provide a comprehensive characterization of the Golgin family protein RUD3 in plant pathogenic fungus, which could help to identify a new potential target for effective control of this devastating disease.
In this study, the design optimisation of a switched reluctance machine (SRM) with the layered method has been studied. Firstly, a multi-physical analytical model for the SRM is established. The proposed model consists of an electromagnetic model, an electrical model, a loss model, and a thermal model. Then a layered optimisation design method suitable for the SRM is proposed in combination with the multi-physical field simulation model. Taguchi method is used to analyse the influence degree of the main geometric dimensions on the dynamic performance, and the parameters are divided into high-sensitivity parameters (HSPs) and low-sensitivity parameters. The non-dominated sorting genetic algorithm-II algorithm is used to optimise the HSP by the proposed model. The iron loss, average torque, average torque per unit mass, torque ripple and efficiency are taken as the objective functions. Finally, the prototype is manufactured and a testing platform is built. The flux linkage of several special positions of the machine is verified experimentally, and the accuracy of the electromagnetic model in the multi-physical field model is verified. The temperature experiments are carried out on the machine, and the accuracy of the equivalent heat circuit model in the multi-physical field model is verified.
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