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The paper is focused on the investigation of the laminar flow and heat transfer characteristics in the interrupted microchannel heat sink with ribs in the transverse microchambers. A three-dimensional model based on finite volume approach and SIMPLEC algorithm is performed considering entrance effect, conjugate heat transfer, viscous heating and temperature-dependent thermo-physical properties. Five different rib configurations are considered, including rectangular, backward triangular, diamond, forward triangular and ellipsoidal. The present study firstly examines the effect of such ribs on velocity contour, pressure distribution and temperature distribution, further the local pressure drop and heat transfer characteristics in such microchannel heat sinks. Result shows that the ribs in the transverse microchambers can effectively prevent the decline of local heat transfer coefficient along the flow direction. Secondly, the average friction factor and Nusselt number for such microchannel heat sinks have been studied. Results show that for Reynolds number ranging