Background: Glioblastoma is one of the most malignant tumors in the brain with high mortality. In recent years, immunotherapy and targeted therapy show great prospects in the treatments for glioblastoma, whereas more effective therapeutic targets are still urgently needed to be developed. Nucleobindin-2 (NUCB2) is the precursor protein of nesfatin-1, which have a variety of metabolic functions, such as food intake and temperature regulation. In recent years, the potential link between NUCB2 and the development of multiple cancer was gradually revealed; however, the effects of NUCB2 on the progression of glioblastoma are still unclear. Methods: Immunohistochemical assays were performed to explore the NUCB2 expression levels in 94 samples of glioblastoma and corresponding nontumor tissues; patients were divided into NUCB2 high expression group and low expression group. Clinical analysis related to the clinical features, the potential link between NUCB2 expression levels, and clinical features were analyzed; the effects of NUCB2 on cell proliferation and invasion of glioblastoma were detected through colony formation and MTT assay, and transwell assay respectively. The possible effects of NUCB2 on tumor growth and metastasis were measured in mice. Results: In this study, we demonstrated that NUCB2 over-expression was correlated with the high degree of recurrence of patients with glioblastoma. Further, we also revealed that NUCB2 promoted cell proliferation and invasion of glioblastoma in vitro and promoted the growth and metastasis of glioblastoma in mice. Conclusion:This study provided evidence that NUCB2 might be a novel therapeutic target of glioblastoma.

Twenty six football players of the Shandong Luneng youth team (U17), the 2019 season Chinese National Youth Super League champions. Based on a comprehensive assessment of the working capacity and func- tional support of intermittent endurance, the levels and changes in the reaction of the cardiorespiratory system and energy supply of football players’ activities were analyzed under the condition of fatigue cu- mulating in the process of performing a series of high-speed segments. The prerequisite for testing was the preservation of the parameters of running 70 m in 10 s during the entire testing period. Content of Cardiorespiratory System and Intermittent Endurance test: duration of work in a series – 4 minutes; dura- tion of work with the maximum available intensity – 10 s (running distance 70 m), rest interval – 20 s. The number of combinations of work segments 10 s and rest intervals 20 s in a series is 8. The efficiency of work is assessed by the number of completed series and the reaction of the cardiorespiratory system. The typo- logical differences in the functional support of the special working capacity of football players who had a high level of working capacity were determined. They are associated with a balanced type of aerobic and anaerobic energy supply, with an insignificant tension of the mechanisms for fatigue compensating and a predominantly anaerobic type of energy supply provided a high level of respiratory compensation for met- abolic acidosis.

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Background: Glioblastoma (GBM) is widely known as a classical kind of malignant tumor originating in the brain with high morbidity and mortality. Targeted therapy has shown great promise in treating glioblastoma, but more promising targets, including effective therapeutic targets, remain to be identified. 18A (KIF18A) is a microtubule-based motor protein that is dysregulated and involved in the progression of multiple human cancers. However, the possible effects of KIF18A on GBM progression are still unclear.Methods: We performed DEG analysis, medical data analysis, and network analysis to identify critical genes affecting glioma progression. We also performed immunohistochemical analysis of the KIF18A levels in 94 patients with glioblastoma and the associated surrounding tissues. Patients were divided into two groups according to the high and low expression. Using a clinical analysis, we showed the potential associations between KIF18A expression and clinical characteristics of 94 GBM patients. We then investigated the effects of KIF18A on GBM cell proliferation by colony establishment, MTT, and immune blogging. The possible effect of KIF18A on GBM tumor growth was determined in mice.Results: We identified KIF18A as a potential gene affecting GBM progression. We further demonstrated that GBM tissues expressed KIF18A much higher, and its presentation was associated with recurrence in glioblastoma patients. We believe KIF18A promotes GBM cell proliferation.Conclusion: We demonstrated that KIF18A could be a promising target in treating GBM.

According to the competition calendar, a one-cycle training process for highly skilled mini-football players in the annual macrocycle was selected. Participants: 15 players of the First League of Ukraine in the futsal mini-football team “Fortetsya” (Kamianets-Podilskyi City), among them 1 player – Honored Master of Sports, 1 – Master of Sports of International Class, 4 – Candidate to Master of Sports, 9 – players of 1st rank. In the competitive period, non-specific means of highly skilled players in mini-football amounted to 40.2%, specific means - 59.8%, including special-prepa- ratory – 6.6%, leading – 30.9% and competitive – 22.3%. Aerobic loads ranged from 40.3 (4-day inter-cycle micro-cy- cle) to 53.6% (4-day competitive micro-cycle) during competitive and inter-gaming micro-cycles of this period. The use of mixed (aerobic-anaerobic) loads in the competitive period of futsal players training ranged from 45.0 (7-day inter-game micro-cycle) to 53.1% (4-day inter-game micro-cycle). The ratio of training means during the competitive period, the volume of training loads of different orientation in micro-cycles of competitive mezocycles of prepara- tion, the ratio of training loads of different orientation: aerobic (49.2%); mixed (46.1%); anaerobic alactatic (2.4%); anaerobic glycolytic (2.3%) have been identified. Taking into account the structure and content of a single-cycle variant of training process structure for the players of the first league team of Ukraine, the volumes of training loads of different orientation, the ratio of the training means of the players in the competitive period were determined.