RESUMO -A maioria dos plantios de bananeira são realizados utilizando mudas tradicionais do tipo chifre e rizomas. Outros métodos de propagação, como a micropropagação, vêm sendo desenvolvidos e aperfeiçoados com fim a elevar a taxa de multiplicação em curto espaço de tempo e melhorar a qualidade fitossanitária das mudas. Contudo, a contaminação por bactérias e fungos é um dos maiores problemas dessa técnica. O objetivo do presente trabalho foi avaliar a desinfestação in vitro utilizando concentrações de cloro ativo em explantes de bananeira 'Farta Velhaco'. O delineamento experimental utilizado foi inteiramente casualizado constituído de cinco tratamentos e cinco repetições, sendo cada uma destas representada por cinco explantes em diferentes concentrações de cloro ativo durante vinte minutos, quais sejam: T1 -testemunha sem cloro ativo; T2 -0,5% de cloro ativo; T3 -1,0% de cloro ativo; T4 -1,5% de cloro ativo; e T5 -2% de cloro ativo. Foram avaliadas as porcentagens de contaminação por bactérias e fungos, como também a porcentagem de oxidação dos explantes. Os dados foram submetidos às análises de variância e de regressão. Os resultados permitiram concluir que a maior eficiência dentre os tratamentos testados foi o de imersão dos explantes em 1% de cloro ativo, o qual proporcionou redução em 95% e 90%, respectivamente, para bactérias e fungos e 60% dos explantes oxidados. Concluiu-se que essa concentração pode ser utilizada para o controle de contaminações para micropropagação de bananeira ´Farta Velhaco'.Palavras-chave: Musa sp.. Micropropagação. Estabelecimento. DESINFESTATION IN VITRO OF BANANA 'FARTA VELHACO' (SUB GROUP AAB)' DIFERENTS CONCENTRATIONS ATIVE CHOROABSTRACT -Most banana plantations are still made using traditional clones per type horn and rhizomes. Other propagation methods such as micropropagation have been developed and perfected to raise the rate of multiplication in a short time and improve the health status of the seedlings. However, contamination by bacteria and fungi is one of the biggest problems of this technique. The aim was to evaluate the in vitro disinfestation using active chlorine concentrations of banana explants 'Farta Velhaco'. The experimental design used was completely randomized consisting of five treatments and five repetition, each replicate represented by five explants in different concentrations of active chlorine for twenty minutes: T1 -without active chlorine, T2 -0.5% active chlorine, T3 -1.0% of active chlorine, T4 -1.5% active chlorine and T5 -2% active chlorine. The percentages of contamination by bacteria and fungi as well as the percentage of oxidation of the explants were evaluated. Data were subjected to analysis of variance and regression analysis. The results showed that the highest efficiency among the tested treatments was the immersion of explants in 1% of active chlorine that caused a reduction in 95% and 90% respectively for bacteria and fungi and 60% of explants oxidation. It was concluded that this concentration can be used to control contamination f...
Propagation methods such as in vitro micropropagation have been developed and improved to increase the multiplication rate in a short time and improve the seedlings quality. The objective of this study was to evaluate the efficiency of the different BAP concentrations on micropropagation of ‘Farta Velhaco’ banana during in vitro multiplication phase. The treatments were: T1 – control (0 mg L-1); T2 - 0.5 mg L-1; T3 - 1 mg L-1; T4 - 1.5 mg L-1; T5 - 2 mg L-1; T6 - 2.5 mg L-1; T7 - 3.0 mg L-1; T8 - 3.5 mg L-1 and T9 - 4.0 mg L-1 of BAP added to the MS culture medium. The total number of shoots produced per explant initial subculture every 30 days was evaluated. The highest number of shoots is recorded 2.5 mg L-1 BAP concentration, which promotes 3 shoots per explant in the 4th subculture. The 4.0 mg L-1 BAP concentration reduces the number of shoots.
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