The objective of the present study was to evaluate the larvicidal potential of the a-amylase inhibitor from the seeds of Macrotyloma uniflorum (Lam.) Verdc. against Aedes aegypti (L.) larvae. Macrotyloma uniflorum a-a.mylase inhibitor (MUAI) was partially purified using ion exchange chromatography on a carboxymethyl cellulose column. MUAI at concentrations of 20, 60, 100 and 200 ppm was tested against different instar larvae, pupae and adults of A. aegypti to establish its bioefficacy. MUAI showed a strong larvicidal and a moderate adulticidal effect at a concentration of 200 ppm but did not exhibit pupicidal and ovicidal activities. MUAI-treated larvae showed lower a-amylase activity. MUAI also exhibited ovipositional deterrence activity. Dosedependent mortality was observed, being highest in the first and second instar larvae. The results thus obtained provide a safe and eco-friendly means in the biological control of A. aegypti larvae.
The current aimed to formulate herbal shampoo and to evaluate the various property of herbal shampoos. The herbal shampoo was formulated by adding the extracts of Spinacia Oleracea leaves, Ipomoea batatas roots, Acacia concinna, Sapindus Indica, Cymbopogon citrus in different proportions to a 100 ml. Small amount of gelatin and citric acid were also added for viscosity and pH respectively. Several tests such as visual inspection, pH, dirt dispersion, rheological evaluation and skin irritation test etc, were performed to determine the physicochemical properties of prepared shampoos. The formulated herbal shampoo was clear and appealing. It showed good cleansing and detergency and good foam stability. The results indicated the formulated shampoo is having excellent conditioning performance, similar to commercially available shampoo. However, further research and development is required to improve its quality and safety.
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