In lentil crop, it was shown that hybridizations between
subspecies macrosperma–microsperma can generate
transgressive segregants for seed yield; however, for antinutritional
and industrial traits, this was not studied yet. The aim of this study
was to identify macrosperma and microsperma lentil genotypes with superior agronomic, industrial, and nutritional
traits that can be used as parents in lentil breeding integral programs
to generate transgressive segregants. Fifteen traits were evaluated
in 21 lentil genotypes in two environments and treatments. ANOVA,
estimation of genetic parameters, and multivariate analysis were performed.
Genotype–environment interactions and variations between genotypes
and environments were significant. Genotypic coefficients of variation
and heritability were high for all traits. With the multivariate analysis,
six macrosperma and three microsperma genotypes with high yield, protein and phenol content, water absorption
and low tannins, phytic acid content, and cooking time were selected
to include in hybridization programs in order to obtain transgressive
segregants.
In pea breeding is important to shorten the generation cycles to obtain homozygosity quickly. Doubled haploid technology is important to attain this purpose and androgenesis is the most promising tool for induction of haploids in legumes. Commercial pea varieties have been described as recalcitrant to this approach but very little is known regarding the androgenic competence of pea relatives. In this work, a comparative study of the androgenic response among different taxa of the genus Pisum was undertaken. We cultured anthers of 11 pea materials from the primary and secondary genepools under the same experimental conditions, and studied their competence to produce calli and plants in vitro. Significant differences were found in the percentage of callus and plant production between the different species and subspecies. The two wild forms Pisum fulvum Sibth. & Sm. and Pisum sativum subsp. elatius (Bieb.) Aschers. & Graebn. regenerated shoots from anther culture with the highest efficiency (67% and 38%, respectively), becoming potential sources of androgenic competence. Among the cultivated genotypes of P. sativum, the botanical variety arvense regenerated shoots with the highest percentage (40%) also being a good candidate to study androgenesis. The commercial varieties tested showed significant differences in the callus and plant production, with Primogénita (FCA-INTA) and B101 giving the best results although with low plant regeneration percentages (17% and 11%, respectively). P. fulvum, P. sativum subsp. elatius and P. sativum subsp sativum var arvense were identified as highly responsive to anther culture, useful to transfer androgenesis competence to recalcitrant commercial varieties.
Key messageWithin genus Pisum, the wild forms P. fulvum, P. sativum subspecies elatius and the cultivated variety arvense were identified as potential sources to introduce androgenesis competence into recalcitrant commercial varieties.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.