Until now, no literature exists to explain about the impact of time, and environmental parameters, when using typical commercial grade transparent linear low density polyethylene (LLDPE) packaging for protecting vegetable cooking oil during typical shelf-life conditions. We tested long-term impact of temperature and illuminance (light: 100 to 150 lux and darkness <1 lux) on refined bleached deodorized palm olein (RBDPOL), fortified with vitamin A. Results showed degradation of fortified RBDPOL with 70 ppm vitamin A was  approximately 15% at 18-22oC in Light, 10% at 18-22oC in Darkness, 19% at 32-33oC in light and 17% 32-33oC in darkness. Similar trend was observed at 45 ppm vitamin A. Exposure to heat and light impacted vitamin A degradation, but is primarily attributed to temperature, irrespective of packaging. This investigation builds on our previous work (Silalahi et al., 2017), and confirms the impact of challenging environmental conditions on vegetable cooking oil shelf-life over long time periods.

Observations about the impact of environmental parameters and time, on fortified vegetable oil, using typical commercial grade linear low density polyethylene (LLDPE) packaging during typical shelf-life conditions, are minimal. We tested the long-term (12 months) impact of temperature and illuminance (light: 100 to 150 lux and darkness <1 lux) on refined bleached deodorized palm olein (RBDPOL), fortified with vitamin A, and without antioxidant. Results showed degradation of fortified RBDPOL with 70 ppm vitamin A was  approximately 19% at 18-22oC in Light, 18% at 18-22oC in Darkness, 38% at 32-33oC in light and 24% 32-33oC in darkness. A similar trend was observed at 45 ppm vitamin A. Exposure to both heat and light impacted vitamin A degradation, but was primarily attributed to prolonged exposure at elevated temperature, irrespective of the packaging material. This short study validates extensive research by Silalahi et al., (2017), doi:10.1111/ijfs.13462 on the impact of environmental effects of fortified vegetable frying oil, during extensive storage life periods.

Currently, in the analysis of triacylglycerol (TAG) composition of the Moringa oleifera seed oil (MOSO), there are still approximately 13.9% of TAG still unknown. Reverse-phase high-performance liquid chromatography (HPLC) coupled to a refractive index detector (RID) has previously been applied for the identification of TAG in MOSO. A new approach focuses on the properties of the fatty acid assay and the full characterisation of TAGs. Identification and determination of TAG compositions in MOSO were done by LC-Q-TOF (LCMS-9030) with ESI interface and HCOONH 4 (ammonium formate) additive in the mobile aqueous phase. Triolein (OOO) was 39.3% and is the dominant TAG in MOSO. This new method was able to fully characterise a total of 33 TAG compositions using accurate mass MS data and MS/MS spectra.

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