In this study, we developed a novel selective method for copper quantification based on gold nanoclusters (GNCs) and DNAzyme.
Pontibacillus litoralis sp. nov., a facultatively anaerobic bacterium isolated from a sea anemone, and emended description of the genus Pontibacillus , was isolated from a sea anemone (Anthopleura xanthogrammica) collected from the South China Sea. Strain JSM 072002T was able to grow with 0.5-15 % (w/v) NaCl and at pH 6.0-10.0 and 15-50 6C;optimum growth was observed with 2-5 % (w/v) NaCl and at pH 7.5 and 35 6C. mesoDiaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C 15 : 0 and anteiso-C 15 : 0 . The predominant respiratory quinone was menaquinone 7 and the genomic DNA G+C content was 41. ). An emended description of the genus Pontibacillus is also presented.The genus Pontibacillus was first proposed by Lim et al. (2005a) and was defined as containing Gram-positive, moderately halophilic, strictly aerobic, motile, endosporeforming rods with meso-diaminopimelic acid cell-wall type and menaquinone 7 (MK-7) as the predominant respiratory quinone (Lim et al., 2005a, b). At the time of writing, the genus comprised three species with validly published names: Pontibacillus chungwhensis (Lim et al., 2005a), Pontibacillus marinus (Lim et al., 2005b) and Pontibacillus halophilus (Chen et al., 2009b). During an investigation of the diversity of the microbial population of invertebrates inhabiting the South China Sea (Chen et al., 2009a, b, c, d, e;Xiao et al., 2009), a facultatively anaerobic, moderately halophilic, endospore-forming, Gram-positive bacterium, designated JSM 072002 T , was isolated from a sea anemone collected from a tidal flat of Naozhou Island, China. Based 3These authors contributed equally to this work.The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strain JSM 072002 T and Pontibacillus marinus KCTC 3917 T are EU583724 and FJ808613, respectively.A supplementary figure showing maximum-parsimony and maximumlikelihood phylogenetic trees based on 16S RNA gene sequences is available with the online version of this paper.
A Gram-positive-staining, moderately halophilic, strictly aerobic, catalase-and oxidase-positive, rod-shaped bacterium, designated strain JSM 076056 T , was isolated from a sea urchin collected from the South China Sea. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 076056 T was able to grow at salinities of 2-25 % (w/v) total salts and at pH 6.0-10.0 and 15-40 6C; optimum growth was observed with 5-10 % (w/v) total salts and at pH 7.0-8.0 and 25-30 6C. It was incapable of growing with NaCl as the sole salt. meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The major cellular fatty acids were iso-C 15 : 0 , iso-C 16 : 0 , iso-C 14 : 0, anteiso-C 15 : 0 and C 16 : 1 v7c alcohol. The predominant respiratory quinone was MK-7 and the genomic DNA G+C content was 45.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 076056 T belonged to the family Bacillaceae and was related most closely to the type strains of the two recognized species of the genus Pontibacillus, namely Pontibacillus chungwhensis BH030062 T (96.4 % sequence similarity) and Pontibacillus marinus BH030004 T (96.2 %); these three strains formed a robust cluster in the phylogenetic tree. In combination, the phylogenetic, phenotypic and chemotaxonomic data indicate that strain JSM 076056 T represents a novel species of the genus Pontibacillus, for which the name Pontibacillus halophilus sp. nov. is proposed. The type strain is JSM 076056 T (5CCTCC AA 207029 T 5DSM 19796 T 5KCTC 13190 T ).The genus Pontibacillus was first proposed by Lim et al. (2005a). Members of this genus are Gram-positive, moderately halophilic, strictly aerobic, motile, endosporeforming rods, having a meso-diaminopimelic acid cell-wall type and containing MK-7 as the predominant respiratory quinone. At the time of writing, the genus comprises two recognized species, Pontibacillus chungwhensis (Lim et al., 2005a) and Pontibacillus marinus (Lim et al., 2005b).During an investigation of the diversity of the microbial population of invertebrates inhabiting the South China Sea, we isolated a moderately halophilic bacterium, designated JSM 076056 T , from a sea urchin collected from Leizhou Bay, China. On the basis of the results of a polyphasic taxonomic study, this strain represents a novel species of the genus Pontibacillus.For strain isolation, serial dilutions (1 : 10) of a sea urchin homogenate were plated on Difco marine agar 2216 (MA; pH 7.5), supplemented with 0-20 % (w/v) NaCl, at 28 u C for 7-28 days. A yellow-pigmented colony, designated strain JSM 076056 T , was picked from a plate of MA supplemented with 10 % (w/v) NaCl. It was maintained both on slants of MA supplemented with 5 % (w/v) NaCl (containing approximately 7 % NaCl and 8.4 % total salts; here referred to as MA5) at 4 u C and in Difco marine broth 2216 (MB) supplemented with 20 % (v/v) glycerol at 280 u C. Unless indicated otherwise, morphological and physiological studi...
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