Objective: The purpose of this retrospective investigation was (1) to screen the existence of HCMV in pancreatic cancer tissues in relation to the histopathological grading system of such tumor tissues. (2) To evaluate the expression of the (P63) tumor suppressor gene in these tissues. (3) To find out the impact of the coexistence of (HCMV) along with the p63 on the occurring histopathological alterations. Methods: The current retrospective cohort study included 35 paraffinized pancreatic tissues from the archives of major hospitals and numerous private histopathological laboratories from 2015 to 2020. (Twenty-five pancreatic carcinomatous tissues and 10 biopsies from seemingly normal pancreatic tissues were examined). Tissue slices from the desired tissue blocks were subjected to the immunohistochemistry (IHC) technique to detect Human Cytomegalovirus pp71 and tumor suppressor P63 proteins with aid of monoclonal primary antibodies. Results: The HCMV pp71 proteins were found in 92% (23 out of 25) of pancreatic tumor tissues, while it was in two (20%) of healthy pancreatic tissues. in comparison, the p63 proteins were found in 76% (19 out of 25) of tumor tissues and in four (40%) of their correlative healthy tissues. Conclusion: The increased expression of HCMV in malignant pancreatic tissue may indicate its primary or secondary role in the emergence of this type of cancer, whereupon HCMV inactivation may be useful in the treatment of this type of cancer. On the other hand, p63’s high levels of expression in malignant pancreatic tumors reflect either an oppressive function or an unfortunate mutation that prevents it from functioning.
Introduction and Aim: Embryo implantation and a healthy placenta depend on the uterine cavity and endometrium, thus any reproductive surgery for unexplained infertility must evaluate these. Hysteroscopy cures intrauterine adhesions, submucous fibroids, endometrial polyps, and endometriosis and directly visualizes the uterus cavity. This study investigated whether hysteroscopy could diagnose uterine disorders in women with recurrent, unexplained miscarriages and improve reproductive outcomes. Methods: This observational retrospective study included 58 patients who had recurrent pregnancy loss and were hospitalized in the Kamal AL-Samarrai Hospital's reproduction unit / Physiopathology section in Baghdad, Iraq between January 2021 and February 2022. The participants were divided into two groups based on whether they had received hysteroscopic surgery. IVF was performed on patients who underwent hysteroscopy surgery. A comparative analysis was made for clinical factors and uterine pathology among the two groups. Data were subjected to statistical analysis. Results: The patients in this study ranged in age from 20 to 42 years, with an average of 3265 years, and a BMI ranging from (20-39) with an average of 29.270.48 kg/m2. Out of the 58 women analyzed, 31 (53.4%), 13 (22.4%), and 48 (82.7%) had a previous cesarean, laparoscopy, and HSG procedures, respectively. 28 patients (48.2%) who underwent hysteroscopic surgery were detected with uterine disorders; IUA (28%), fibroids (25%), submucosal polyps (21.4%), endometrial hyperplasia (17.8%), and endometriosis (7.1%). No significant difference was seen for the duration of infertility, type of infertility, or previous IVF attempt failure between the hysteroscopic and control groups (p>0.05). In women who underwent IVF procedures after hysteroscopic surgery, the clinical pregnancy rate was lower as compared to women without surgery. Conclusion: Hysteroscopy can diagnose some intrauterine diseases early enough for surgery. Undiagnosed infertility in women with RPL suggests a hysteroscopic examination before in vitro fertilization.
Purpose: Total antioxidant capacity (TAC) in women serum and follicular fluid (FF) which surrounding oocytes may be related to the implantation failure .Therefore, we herein examined the relationship between total antioxidant capacity status in serum and FFand it is association with implantation failure Method: One hundred and seventeen of non-reproductive women who underwent intra-cytoplasmic sperm injection (ICSI)included in this study and conducted between March 2018 and April 2019 in Kamal AL-Samarai Hospital, center of fertility and IVF. Serum and follicular fluid were collected from non-reproductive women aged ranged 20-45 years and BMI (ranged 21.9-36.3kg/m 2 ), TAC were measured using sandwich ELISA in serum and follicular fluid specimen of 21 women of successful implantation compared to 96 experienced implantation failures.Results: TAC was increased in serum of implantation failure compared to successful but not significant differences between two study groups. Whereas TAC levels were highly significant (P=0.002) inFF of women who had successful implantation (1.08 ± 0.64mmol/L) whereas in failure were lower(0.55 ± 0.42 mmol/L). In addition to that, when evaluating the frequency of TAC category in FF revealed highly significant differences (P=0.003) between two groups, the majority of failure groups(84.4%) had low TAC compared with (40%) in successful groups, while a significant increase of sufficient TAC in successful than failure groups (50% versus 6.1% respectively), whereas the borderline TAC were (9.1% versus 10%) in failure and successful groups.Conclusions: TAC in FF may be potential markers for implantation successful in ICSI cycle.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.