Host individuals and populations often vary in their responses to infection, with direct consequences for pathogen spread and evolution. While considerable work has focused on the mechanisms underlying differences in resistance-the ability to kill pathogens-we know little about the mechanisms underlying tolerance-the ability to minimize fitness losses per unit pathogen. Here, we examine patterns and mechanisms of tolerance between two populations of house finches (Haemorhous [formerly Carpodacus] mexicanus) with different histories with the bacterial pathogen Mycoplasma gallisepticum (MG). After infection in a common environment, we assessed two metrics of pathology, mass loss and eye lesion severity, as proxies for fitness. We calculated tolerance using two methods, one based on pathology and pathogen load at the peak of infection (point tolerance) and the other based on the integrals of these metrics over time (range tolerance). Alabama birds, which have a significantly longer history of exposure to MG, showed more pronounced point tolerance than Arizona birds, while range tolerance did not differ between populations. Alabama birds also displayed lower inflammatory cytokine signaling and lower fever early in infection. These results suggest that differences in inflammatory processes, which can significantly damage host tissues, may contribute to variation in tolerance among house finch individuals and populations. Such variation can affect pathogen spread and evolution in ways not predictable by resistance alone and sheds light on the costs and benefits of inflammation in wild animals.

The ethical and logistical challenges inherent in experimental infections of wild-caught animals present a key limitation to the study of wildlife diseases. Here we characterize a potentially useful domestic model for a wildlife disease that has been of particular interest in recent decades; that is, infection of North American house finches (Carpodacus mexicanus) with Mycoplasma gallisepticum, more commonly known as a worldwide poultry pathogen. Seven domestic canaries (Serinus canaria domestica) were infected experimentally with M. gallisepticum alongside two wild-caught house finches (C. mexicanus) and the resulting clinical disease, pathogen load, serology and pathology were compared. Although rates of morbidity were higher in domestic canaries in response to M. gallisepticum infection, no significant differences were detected between the two species in the four measures of infection and disease studied. Our results support previous field and experimental studies that have documented universal susceptibility to M. gallisepticum infection in the avian family Fringillidae, which includes domestic canaries. Our results also indicate that domestic canaries may serve as a potentially useful model system for the experimental study of M. gallisepticum infection in songbirds.

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The Winter Food Limitation Hypothesis (WFLH) states that winter food abundance is a dominant source of population limitation of migratory birds. Evidence is accumulating that long-distance migratory birds wintering in tropical climates have high overwinter survival probabilities and that winter food limitation mainly affects their fitness nonlethally by limiting energetic reserves necessary for successful reproduction. In contrast, the relative roles of direct mortality vs. indirect effects caused by food limitation have not been investigated thoroughly on short-distance migratory birds wintering in temperate zones, where they face thermal challenges. We performed the first test of the WFLH for a temperate migratory bird in the wild on the Swamp Sparrow (Melospiza georgiana), with a replicated plot-wide food supplementation experiment. In contrast to tropical, but consistent with other temperate-wintering migrants, Swamp Sparrows on unmanipulated plots showed relatively low apparent survival across the winter. Following food addition, birds (1) immigrated to experimental plots, which subsequently supported approximately 50% higher abundances, (2) experienced increases of within-season apparent survival of 8-10%, depending on age/sex class, and (3) had higher-scaled mass index values, all supporting winter food limitation. The last two findings are interrelated because birds with higher scaled mass had higher survival probabilities, further supporting direct effects of winter food limitation. Food limitation of fat reserves might also have indirect effects on reproductive success by limiting migration timing and survival during migration. Increases in scaled mass were higher in females, suggesting that they are disproportionately affected by food limitation, possibly through competition. Based on Robust Design Modeling, we found no support for emigration prior to food addition, indicating that our estimates of mortality are unbiased.

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