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Biodegradable alpha-tricalcium phosphate/iron (α-TCP/Fe) composites are promising candidates for the fabrication of temporal osteosynthesis devices. Similar to biodegradable metals, these composites can avoid implant removal after bone fracture healing, particularly in young patients. In this work, α-TCP/Fe composites are studied for the first time in a wide range of compositions, showing not only higher degradation rate in vitro than pure components, but also good cytocompatibility and mechanical properties controllable with the Fe content. Ceramic matrix composites show high specific strength and low elastic modulus, thus better fulfilling the requirements for bone fractures fixation. A significant advance over previous works on the topic is the use of pulsed electric current assisted sintering together with α-TCP, convenient to improve the mechanical performance and degradation rate, respectively.

In this work, large 3D Ti meshes fabricated by direct ink writing were wirelessly anodized for the first time to prepare highly photocatalytically active TiO 2 nanotube (TNT) layers. The use of bipolar electrochemistry enabled the fabrication of TNT layers within the 3D Ti meshes without the establishment of an electrical contact between Ti meshes and the potentiostat, confirming its unique ability and advantage for the synthesis of anodic structures on metallic substrates with a complex geometry. TNT layers with nanotube diameters of up to 110 nm and thicknesses of up to 3.3 μm were formed. The TNT-layer-modified 3D Ti meshes showed a superior performance for the photocatalytic degradation of methylene blue in comparison to TiO 2nanoparticle-decorated and nonanodized Ti meshes (with a thermal oxide layer), resulting in multiple increases in the dye degradation rate. The results presented here open new horizons for the employment of anodized 3D Ti meshes in various flow-through (photo)catalytic reactors.

In this work we investigate the relationship of the F line and CaF bands with varying Ca and F contents.

Purpose Materials with a high thermal conductivity, such as Cu-alloys hold the most interest to the plastic moulding industry. Additive manufacturing (AM), especially selective laser melting (SLM) of metals, allows the production of parts with complicated internal cooling and increased production efficiency. The portfolio of alloys for metal AM is limited and still missing process parameters for the processing of copper alloys. This paper aims to preview the process parameters of high-strength alloy Cu7.2Ni1.8Si1Cr processed by SLM. Design/methodology/approach An experimental approach is adopted to investigate porosity and mechanical properties of SLM specimens and its comparison with standard material AMPCOLOY 944. Optimization of porosity was performed using line and cube specimens; mechanical properties and microstructure were evaluated by tensile testing and metallography. Findings Optimum processing parameters for fabrication of Cu-alloy specimens with a relative density of 99.95 per cent were identified, and no cracks were detected. Mechanical testing of SLM specimens showed the ultimate tensile strength, proof stress of 0.2 and elongation of 380, 545 MPa and 16.9 per cent. The alloy is suitable for laser AM, thanks to its processability at a relatively high laser scanning speeds and thus its promising price of part/costs ratio. Research limitations/implications The paper describes the initial state of research – the follow-up tests focussed on mechanical testing, fatigue and statistical evaluation need to be conducted. The process parameters are developed only for bulk geometry – optimal setup for lattice structures and thin walls has not been explored yet. Practical implications The research findings in this work could be used for production of 3D printed parts and after the tuning of additional parameters, e.g. for up- and down-skin zones, could be used for special application such as energy exchange. Originality/value This work produces the processing of new material suitable for laser AM. Cu7.2Ni1.8Si1Cr alloy could be the prospective material from the group of Cu alloys suitable for moulds manufacturing and thermal applications.

Biofabrication and maturation of bone constructs is a long-term task that requires a high degree of specialization. This specialization falls onto the hierarchy complexity of the bone tissue that limits the transfer of this technology to the clinic. This work studied the effects of the short-term cryopreservation on biofabricated osteoblast-containing structures, with the final aim to make them steadily available in biobanks. The biological responses studied include the osteoblast post-thawing metabolic activity and the recovery of the osteoblastic function of 3D-bioprinted osteoblastic structures and beta tricalcium phosphate (β-TCP) scaffolds infiltrated with osteoblasts encapsulated in a hydrogel. The obtained structures were cryopreserved at −80 °C for 7 days using dimethyl sulfoxide (DMSO) as cryoprotectant additive. After thawing the structures were cultured up to 14 days. The results revealed fundamental biological aspects for the successful cryopreservation of osteoblast constructs. In summary, immature osteoblasts take longer to recover than mature osteoblasts. The pre-cryopreservation culture period had an important effect on the metabolic activity and function maintain, faster recovering normal values when cryopreserved after longer-term culture (7 days). The use of β-TCP scaffolds further improved the osteoblast survival after cryopreservation, resulting in similar levels of alkaline phosphatase activity in comparison with the non-preserved structures. These results contribute to the understanding of the biology of cryopreserved osteoblast constructs, approaching biofabrication to the clinical practice.

Direct ink writing is a popular method for fabrication of scaffolds, yet its widespread usage in clinical practice requires guarantee of compatibility of a scaffold with bone tissues. Mechanical compatibility is mandatory to prevent stress shielding and is expressed using the difference between effective elastic constants (EECs) of a scaffold and a tissue. In this paper, the solution for EECs of a calcium phosphate scaffold is derived for any combination of input parameters, inclusive of the contact radius at the joints, a feature arising mainly from the rheological properties of the ink. The model was validated by comparison with data from the literature and those obtained from tests on produced scaffolds and monoliths. The contact radius significantly influences elastic response, especially for small overlap between the printed layers. The inverse solution can be used for estimation of bulk properties but is also helpful for quality assessment of the fabrication process.

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