Introduction: Diabetes mellitus and its complications are spreading with increased rate in Asian population especially in Pakistan. Uncontrolled diabetes can lead to different micro vascular complications. Cataract is one of the complications of diabetes which may lead to lens degenerative changes and visual impairment. G6PD plays a vital role in preventive measurements from cataract development in normal population.
Objectives: This Study was designed to estimate G6PD levels in diabetic without ocular manifestations & diabetic cataract population.
Methodology: This cross sectional comparative study was done at the Department of Biochemistry LUMHS Jamshoro in collaboration with the Diabetic clinic, Institute of Ophthalmology & Diagnostic Research Laboratory LUMHS Jamshoro. 100 diagnosed subjects of diabetes were selected by Non-Probability type of sample technique with consent of subjects and they were divided in to two groups Group A as control 50 diabetic subjects with out ocular manifestation while Group B as case study group contain 50 subjects of diabetes with cataract. The fasting blood glucose level was estimated by Hexokinase Method while G6PD level was measured by kit method on SD Biosensor while HbA1c(% ) was estimated by TTAB methodology. The data was statistically analyzed by SPSS 21.
Results: The mean level of G6PD in Group A was 15.63±2.45 u/Hb while in group B it was 9.01±3.11 u/G HB. This result finally concluded that there was significantly (<0.05) decline of G6PD diabetic cataract as compared with diabetic without cataract.
Conclusion: This study concluded that there was significant decline in G6PD level in diabetic cataract. It is also concluded that the estimation of G6PD level in diabetic population will be beneficial to take early preventive measurements against diabetic vascular complications.
Background: Diabetes Mellitus is one of the alarming health hazardous indicator and with poor glycemic control, it will be dangerous for human health because it is more prone to development of different systemic & vascular complications. G6PD is one of the carbohydrate metabolic enzyme which prevent from oxidative stress and development of free radicals. Objective: The purpose of this research is to evaluate the level of G6PD in patients of type-2 diabetes mellitus with their different levels of glycemic control index. Methodology: This study was done at Department of Biochemistry LUMHS in collaboration with Diabetic clinic LUMHS and Diagnostic & Research Laboratory LUMHS Jamshoro. Total 100 diagnosed cases of type-2 diabetes were selected and divided into three groups with different categories of glycemic control index. The HbA1c% was measured by Bio Red Variant while G6PD was measured by standard G6PD quantitative measurement method at Diagnostic laboratory LUMHS using assay kit (SD Biosensor, Inc. Republic of Korea). Results: The G6PD significantly (p < 0.05) decline in diabetic patients with poor glycemic control having mean values of HbA1c% between 11-13%. Conclusion: This research study concluded that there is significant decline in G6PD in patients of diabetes mellitus with poor glycemic control. Estimation G6PD also can use as screening test in diabetic population to determine their genetic involvement. Keywords: Type-2 Diabetes Mellitus, HbA1c%, G6PD
Background: Diabetes Mellitus is one of the leading cause of morbidity & mortality all over the world. The poor glycemic control in the patients of type-II diabetes mellitus can lead the development of complications which cause the morbidity in the life of diabetic population. The aim of this study was to estimate the serum sialic acid in the patients of type- II diabetes for early prediction of diabetic complications. Methodology: This case comparative study was conducted at the Diabetes Clinic of LUMHS Jamshoro with the collaboration of the Department of Biochemistry at LUMHS. A total of 84 diagnosed cases of type-II Diabetes Mellitus were included in this study. In group A, 40 diabetes mellitus cases were diagnosed with a history of diabetes less than five years, while in group B 44 had histories of diabetes over ten years. Spectrophotometers were used to measure serum sialic acid levels using Ehrlich's reagent. SPSS version 22 was used for data analysis with independent student t tests applied. Results: The mean serum sialic acid level of group A was 56.42 ± 5.67 mg/dl, while serum sialic acid level of group B was 69.88 ± 7.34 mg/dl. There was significant (p<0.05) increased level of serum sialic acid in the type-II diabetic patients of group B, which indicate there is more chance to development of micro vascular complications in type-II diabetic patients with prolong duration for their diseases period. Conclusion: Estimation of serum sialic acid in the patients of type-II diabetes mellitus is one of the early predictive marker for the complications of diabetes mellitus. Keywords: Type-II Diabetes Mellitus, Serum Sialic Acid, Estimation, HBA1C
Background: Foot infection involving the skin, soft tissues and bony structures, is a common complication of foot ulcers in diabetic individuals and represents a major cause of morbidity and mortality. Systemic antibiotic treatment must be given as early as possible initially on empirical basis and based on the response to empirical therapy and wound culture and sensitivity results, definitive therapy should be decided. This study was conducted to assess the efficacy and safety of moxifloxacin for treating diabetic foot infections in Pakistani population. Methods: After taking ethical approval, an interventional phase IV study was conducted in the department of medicine and diabetic clinic,
Aims & Objective: This study was conducted to estimate the serum magnesium level in patients of type-II diabetes mellitus and diabetic nephropathy. Methodology: This case control study was conducted at LUMHS Jamshoro Sindh Pakistan. Total 120 subjects were selected for this research divided in to three groups, control and two case study groups for the period of six months July 2020 to December 2020. FBS, HbA1%, serum creatinine, serum magnesium levels were estimated from all subjects. The statistical analysis was done by SPSS version 21 by applied ANOVA test to compare the parameters in different groups. Results: Serum Magnesium level was significantly (p<0.05) decline in group C patients of diabetic nephropathy with poor glycemic control as compared to control or diabetic patients without complications. Conclusion: Estimation of serum magnesium level in the diabetic patients can be use for the early predictor tool to know the expansion of diabetic complications. Keywords: Type-2 Diabetes Mellitus, Diabetic Nephropathy, Serum Magnesium
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