Purpose The purpose of this study is to assess outcomes after magnetic-activated cell sorting (MACS) technology on obstetric and perinatal outcomes compared with those achieved after swim up from randomized controlled trial. Methods This is a two-arm, unicentric, prospective, randomized, and triple-blinded trial and has a total of 237 infertile couples, between October 2010 and January 2013. A total of 65 and 66 newborns from MACS and control group, respectively, were described. Results MACS had no clinically relevant adverse effects on obstetric and perinatal outcomes. No differences were found for obstetric problems including premature rupture of membranes 6.1% (CI95% 0-12.8) vs. 5.9% (CI95% 0-12.4), 1st trimester bleeding 28.6% (CI95% 15.9-41.2) vs. 23.5% (CI95% 11.9-35.1), invasive procedures as amniocentesis 2.0% (CI95% 0-5.9) vs. 3.9% (CI95% 0-9.2), diabetes 14.3% (CI95% 4.5-24.1) vs. 9.8% (CI95% 1.6-17.9), anemia 6.1% (CI95% 0-12.8) vs. 5.9%(CI95% 0-12.4), 2nd and 3rd trimesters 10.2% (CI95% 1.7-18.7) vs. 5.9% (CI95% 0-12.4), urinary tract infection 8.2% (CI95% 0.5-15.9) vs. 3.9% (CI95% 0-9.2), pregnancy-induced hypertension 6.1% (CI95% 0-12.8) vs. 15.7% (CI95% 5.7-25.7), birth weight (g) 2684.10 (CI95% 2499.48-2868.72) vs. 2676.12 (CI95% 2499.02-2852.21), neonatal height (cm) 48.3 (CI95% 47.1-49.4) vs. 46.5 (CI95% 44.6-48.4), and gestational cholestasis 0%(CI95% 0-0) vs. 3.9% (CI95% 0-9.2), respectively, in MACS group compared with control group. Conclusions Our data suggest that MACS technology does not increase or decrease Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation adverse obstetric and perinatal outcomes in children conceived when this technology was performed, being the largest randomized control trial with live birth reported results with MACS.
In this review, our aim is to give an overview of the state of the technology and clinical outcomes of time-lapse microscopy in improving embryo selection as a key step in in vitro fertilization (IVF). Using traditional incubators, morphologic assessment of the fertilized embryos is limited to snapshots at a few discrete points in time, reducing the amount of information that could potentially be obtained. Time-lapse monitoring overcomes this limitation without exposing the embryos to environmental changes. Moreover, time-lapse may introduce new dynamic markers of embryo competence as well as a versatile embryo evaluation and provides novel information regarding human embryo development. In the last few years, various algorithms have been developed correlating the kinetics of early embryo development to blastocyst formation, implantation potential, chromosomal content and live birth rate. There is not yet a universally accepted algorithm, and significant knowledge gaps remain that can provide opportunities for further research in this field.
FISH component (p¼0.003). Female age approached significance in both models; each year increased the risk of SAB by 1.2% (p¼0.081). Sperm disomy, male age, female reproductive factors, semen volume, sperm density, and sperm motility did not predict SABs (p>0.05). Abnormal FISH testing did not predict IF (p¼0.259). The overall pregnancy rate in men with abnormal sperm FISH was 0.95% using IUI, 10.2% using IVF, 14.8% using IVF/ICSI, and 50% when preimplantation genetic screening (PGS) was added to IVF.CONCLUSIONS: The incidence of aneuploid abnormalities of chromosomes 13, 18, 21, and the sex chromosomes on sperm FISH testing predicts SABs. Couples with abnormal sperm FISH results should be counseled regarding the potential negative pregnancy outcomes, and IVF with PGS discussed.
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