Obstetric and perinatal outcome of babies born from sperm selected by MACS from a randomized controlled trial

Abstract

Purpose The purpose of this study is to assess outcomes after magnetic-activated cell sorting (MACS) technology on obstetric and perinatal outcomes compared with those achieved after swim up from randomized controlled trial. Methods This is a two-arm, unicentric, prospective, randomized, and triple-blinded trial and has a total of 237 infertile couples, between October 2010 and January 2013. A total of 65 and 66 newborns from MACS and control group, respectively, were described. Results MACS had no clinically relevant adverse effects on obstetric and perinatal outcomes. No differences were found for obstetric problems including premature rupture of membranes 6.1% (CI95% 0-12.8) vs. 5.9% (CI95% 0-12.4), 1st trimester bleeding 28.6% (CI95% 15.9-41.2) vs. 23.5% (CI95% 11.9-35.1), invasive procedures as amniocentesis 2.0% (CI95% 0-5.9) vs. 3.9% (CI95% 0-9.2), diabetes 14.3% (CI95% 4.5-24.1) vs. 9.8% (CI95% 1.6-17.9), anemia 6.1% (CI95% 0-12.8) vs. 5.9%(CI95% 0-12.4), 2nd and 3rd trimesters 10.2% (CI95% 1.7-18.7) vs. 5.9% (CI95% 0-12.4), urinary tract infection 8.2% (CI95% 0.5-15.9) vs. 3.9% (CI95% 0-9.2), pregnancy-induced hypertension 6.1% (CI95% 0-12.8) vs. 15.7% (CI95% 5.7-25.7), birth weight (g) 2684.10 (CI95% 2499.48-2868.72) vs. 2676.12 (CI95% 2499.02-2852.21), neonatal height (cm) 48.3 (CI95% 47.1-49.4) vs. 46.5 (CI95% 44.6-48.4), and gestational cholestasis 0%(CI95% 0-0) vs. 3.9% (CI95% 0-9.2), respectively, in MACS group compared with control group. Conclusions Our data suggest that MACS technology does not increase or decrease Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation adverse obstetric and perinatal outcomes in children conceived when this technology was performed, being the largest randomized control trial with live birth reported results with MACS.

Decrease of spermatozoa with an unbalanced chromosome content after cell sorting in men carrying a structural chromosomal abnormality

“…Moreover in 2017 Romany et al did a randomized control trial on 237 couples with liveborn children conceived through the association of MACS and ICSI. They concluded that there were no noticeable differences in obstetric or perinatal events when MACS is used (Romany et al 2017). However, further research is needed to verify the presence of freely floating microbeads in the annexin V (-) fraction on the plasma membrane of the spermatozoa, and long term effects on the health and development of children.…”

The Effect of Density Gradient Centrifugation and Magnetic-Activated Cell Sorting with the Combination of Both on Bovine Spermatozoa Kinematics, Viability and Acrosome Status

“…They suggested that not all species are suitable for MACS separation method. In our knowledge, most of the research for MACS separation or the combination of DGC and MACS used human samples (de Vantéry Arrighi, Lucas, Chardonnens, & De Agostini, 2009;Gil, Shalom, Sivira, Carreras, & Checa, 2013;Martin et al, 2017;Romany et al, 2017). DGC has been proven to effectively remove nonviable spermatozoa.…”

Magnetic-Activated Cell Sorting (MACS): A Useful Sperm-Selection Technique in Cases of High Levels of Sperm DNA Fragmentation

“…Patients using their own oocytes, and those undergoing oocyte-donation treatment were included. Details of donor selection and work-up studies were previously reported [ 20 ].…”

“…This technique also reduces the proportion of sperm with fragmented DNA in the ejaculate before using it for ART procedures [ 19 ]. In addition, there are no clinically adverse effects at the obstetric and perinatal levels in newborns who were closely monitored following oocyte-donation and sperm-selection treatments with MACS [ 20 ].…”