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Ultrasonic velocity ( U ), density ( ρ ), viscosity ( ƞ ) for the ternary mixture of benzene + chloroform + cyclohexane in the whole range of composition has been carried out at various temperatures 303.15, 308.15 and 313.15K. From the measured parameters U, ρ and ƞ , some derived parameters β, L f , V f , π i , Z, R,W with molar enthalpy ( H m ) and apparent molar volume ( ɸ v ) are also estimated. The decreasing trend of ƞ, R,W, H m , and ɸ v with concentrations and other parameters are in increasing trend were observed. The trend of acoustical and physico-chemical parameters confirm the dynamics of molecules at higher temperature and the magnitude of intermolecular interactions among the constituents of the mixtures always reflects the nature of substance. The role of enthalpy and apparent molar volume has been used to determine their thermal response. The sign and magnitude of these properties are evident for the nature of interactions between component molecules.

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Starch metabolism due to adsorption of enzyme amylase on the starch substrate is outlined briefly. To explore the necessary conditions required for effective adsorption in biological media, ultrasonic techniques have been applied to elucidate the structural variations and component destruction in the considered systems. The ability of the enzyme amylase to break the linkages in two different polysaccharide substrates was determined from the observed ultrasonic data, which highlights the deciding factors of metabolism. It is concluded that the phenomenon of adsorption is decided by the surface area, the number of subunits held by the substrate, and the structure existing in the adsorbent, and above all, a relatively higher quantity of enzyme than the substrate.

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