Objective.
To investigate a nasopharyngeal carriage and serotypes of S. pneumoniae in children aged 3 to 6 years from day-care centers in Smolensk.
Materials and Methods.
A total of 245 isolates of S. pneumoniae from 1027 nasopharyngeal swabs from healthy children attending day-care centers was tested. Identification of S. pneumoniae was performed according morphological, cultural and antigenic characteristics (Slidex pneumo-Kit, bioMeriеux, France), susceptibility to optohin and bile. Extraction of S. pneumoniae DNA (338) from nasopharyngeal specimens and cultures was performed using nucleic acids extraction kit AmpliSens® DNA-sorb-В (Interlabservice, Russia). Molecular typing was performed using CDC-recommended PCR method with 22 pairs of primers.
Results.
Children without signs of infection diseases from 14 day-care centers were examined, whose parents have signed an informed consent. There were 245 (24.0%) isolates of S. pneumoniae from 1027 specimens of nasopharynx’s swabs. The partly vaccinated with PCV13 were children aged 3–4 years – 311 (30.3%), in 95 children were healthy carriage (38.2%) of S. pneumoniae. The majority of children under 4 years (716⁄69.7%) were non vaccinated, healthy carriage of S. pneumoniae were 216 (29.3%). The predominant serotypes of S. pneumoniae in partly vaccinated children were: 3 (9.8%), 6AB (17.9%), 19F (13.8%), 11AD (12.2%), 23F (5.3%), 19A (2%), 18ABCF (1.6%), 14 (2%), 9AV (1.2%), 6CD (0.8%). The coincidence of S. pneumoniae serotypes in vaccinated children and included in PCV13 were 54%. The most common serotypes of S. pneumoniae in non-vaccinated children were: 11AD (12.2%), 15A/F (2%), 23A (2%), 22AF (0.4%), 19A (1.3%), 33FA/37 (1.6%) and non-typeable serotypes (27.8%).
Conclusions.
The low coverage of pneumococcal vaccine in children under 6 years shows the initial process of its implementation in Health Care. We detect the reduction of vaccine serotypes in children with incomplete immunization (54%) and notable increase in non-typeable serotypes (27.8%) in nonvaccinated children.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.