the purpose of this work was to study the correlation between oxidative stress (oS) marker and angiotensin converting enzyme (aCe) activity in patients with chronic kidney disease stages I-II (uncomplicated pyelonephritis). the 32 patients with uncomplicated pyelonephritis and 30 healthy volunteers (women, age -18-40 years) R ecently much attention has been paid to the study of renal function in regulating the homeostasis in the assessment of the role of callicrein-kinin, the renin-angiotensin-aldosterone system and prostaglandins, their role in the development of oxidative stress (OS) in chronic kidney disea se (CKD) [1,2]. It is well known that the activation of the callicrein-kinin and renin-angiotensinaldosterone systems causes the increased secretion of vasoactive peptides, including angiotensin II, bradykinin, renin, and promotes the activation of a wide spectrum of neurohormonal, metabolic, inflammatory and procoagulation reactions, that leads to the increase of reactive oxygen species (ROS) [3][4][5][6].The pathogenetic role of angiotensin-II was proved in the development of nephropathy and hypertension. It plays a direct role in the vasopressor effects, promotes intraglomerular hypertension and is one of the key molecules that trigger oxidative stress and inflammation in the vascular wall. One of the effects of angiotensin II is the activation of nuclear transcription factors that stimulates the expression of cytokines, chemokines, nitric oxide (NO), cyclooxygenase-2, adhesion molecules. Endothelial damage primarily affects the production of NO-synthase (NOS) [1,7].Angiotensin-II is involved in the inflammatory reactions and enhances the proliferation of cells, including renal glomerular mesangial cells, contributing to the progression of fibrosis. Another important factor of disorder of renin-angiotensin system is transforming growth factor β (TGFβ), which stimulates synthesis of the various components of the extracellular matrix degradation and blocks protease inhibitors. In addition, TGF-β stimulates the production of endothelin-1 -a powerful vasoconstric-
The aim of the research was to study the effect ofoxidative factors impact and modality of renal replacement therapy (BBT) on indices ofoxidative stress (OS) and resistance of erythrocytes membranes in patients with chronic kidney disease stage V(CKD VD) and anemie. Material and methods. The study involved 68 patients with CKD VD: 14 patients were treated by hemodiafiltration (HDF), 25 patients by hemodialysis (HD) and 29 patients by peritoneal dialysis (PD). The severity ofanemia was assessed according to the KDIGO (2012) criteria. The control group consisted of 30 healthy people of the same age and sex. Along with the standard diagnostic methods, we defined the content of malonic dialdehyde in serum (MDAs) and in erythrocytes (MDAe), the content of ceruloplasmin (CPs), transferrin (TBs) and SH-groups in the blood serum, the index of the OS (IOS), catalase activity in serum (CATs), glucose-6-phosphate dehydrogenase (G-6-PDHe) and total peroxidase activity (TPA) in erythrocyte, peroxide resistance (PR) of red blood cells and erythrocyte membrane permeability (EMP). Statistical analysis was performed using the programs of Microsoft Excel 7.0. Results. It has been stated that in the CKD VD patients in compatison with control group the MDAs content increased by 3.3 times and MDAe - 1.2 times, TBs content reduced by 34%, SH-groups - by 31%, TPAe - by 41% and G- 6-FDGe - by 58%, PB-by 60%; 4.6 times increased CATs activity and OSI; 2 times grew peroxide hemolysis (PH) and 1.3 times - EMP. The analysis (depending on the BBT modality) showed that the patients treated by HDF had typical MDAs increase by 3.9 times on a background of CPs by 24%, TBs - 33%, SH-groups - 25%, TPAe - 51%, G-6-PDHe - 42%; the increase in serum OSI - 5.4 times and 2.6 times in erythrocytes, PB - by 3.6 times and CATs activity by 3.5 times; HD group was characterized by the highest value of MDAe, OSI, PH and CATs, along with more expressed decrease of TBs indices, SH-groups, TPA and G-6-FDHe activity compared with rates in patients with HDF. The patients treated with PD had the lowest content of MDAs and the highest values on the background ofTPAe, the significant increase of CPs by 1.7 times and lowest TBs and G-6-PDHe. The patients with PD showed twice lower OS activity by OSI. Conclusion. Thus, in patients with CKD VD, who had HD, HDF or PD an anemie was associated with high OS activity and the increased degree of hemolysis. These changes are stipulated by BBT methods: for patients receiving HDF were typical the lowest rates of hemolysis and the highest degree ofprotection for erythrocytes, and for patients treated with HD - the highest OS.
Aim of the work was to evaluate the possibility of determining the oxidative stress index (OSI) in blood serum of women with pyelonephritis as prognostic marker of recurrent pyelonephritis.
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