Intensity of oxidative stress and activity of angiotensin converting enzyme in blood of patients with uncomplicated pyelonephritis

Abstract

the purpose of this work was to study the correlation between oxidative stress (oS) marker and angiotensin converting enzyme (aCe) activity in patients with chronic kidney disease stages I-II (uncomplicated pyelonephritis). the 32 patients with uncomplicated pyelonephritis and 30 healthy volunteers (women, age -18-40 years) R ecently much attention has been paid to the study of renal function in regulating the homeostasis in the assessment of the role of callicrein-kinin, the renin-angiotensin-aldosterone system and prostaglandins, their role in the development of oxidative stress (OS) in chronic kidney disea se (CKD) [1,2]. It is well known that the activation of the callicrein-kinin and renin-angiotensinaldosterone systems causes the increased secretion of vasoactive peptides, including angiotensin II, bradykinin, renin, and promotes the activation of a wide spectrum of neurohormonal, metabolic, inflammatory and procoagulation reactions, that leads to the increase of reactive oxygen species (ROS) [3][4][5][6].The pathogenetic role of angiotensin-II was proved in the development of nephropathy and hypertension. It plays a direct role in the vasopressor effects, promotes intraglomerular hypertension and is one of the key molecules that trigger oxidative stress and inflammation in the vascular wall. One of the effects of angiotensin II is the activation of nuclear transcription factors that stimulates the expression of cytokines, chemokines, nitric oxide (NO), cyclooxygenase-2, adhesion molecules. Endothelial damage primarily affects the production of NO-synthase (NOS) [1,7].Angiotensin-II is involved in the inflammatory reactions and enhances the proliferation of cells, including renal glomerular mesangial cells, contributing to the progression of fibrosis. Another important factor of disorder of renin-angiotensin system is transforming growth factor β (TGFβ), which stimulates synthesis of the various components of the extracellular matrix degradation and blocks protease inhibitors. In addition, TGF-β stimulates the production of endothelin-1 -a powerful vasoconstric-

Arylesterase activity of paraoxonase 1 and intensity of oxidative processes in the blood of dialysis patients

“…ПОН-1 інактивує токсичні продукти, які утворюються у результаті окислення ЛПНЩ, стимулюючи відтікання холестерину з макрофагів через ЛПВЩ [27]. ПОН-1 також пригнічує диференціювання моноцитів в макрофаги, тим самим обмежуючи процес утворення пінистих клітин і зменшуючи утворення атеросклеротичних бляшок [20,22,24]. Отже, зниження активності ПОН-1 сприяє уповільненню даних процесів у пацієнтів з ХХН.…”

Взаємозв'язок Між Дозою Тривалого Активатора Рецепторів Еритропоетину Та Оксидативним Стресом У Хворих, Які Лікуються Методом Гемодіалізу

“…In order to estimate MDA, we used serum and red cell suspensions for blood which was drawn using vials containing of 3.8% sodium citrate and centrifuged for 10 minutes at 3000 g [13].…”

“…After 30 minutes incubation at room temperature, absorbance was measured at 440 nm. As a standard, a TR solution was used [13].…”

Age-dependent cardioprotective action of meldonium on heart remodeling under the experimental hypoosmolar hyperhydration

“…In addition, water overload has a city in chronic renal disease, which,in turn, significantly increases the cardiovascular diseases risk 5 . Kidney failure is often an indication for hemodialysis, which only aggravates the hydration 6 .Undoubtedly, the body's response to water-salt balance disorders varies according to age 7 . So probably a complex correction of chronic water overload of the body also requires the prescription of cardioprotectors.…”