SummaryThe aim of this work was to evaluate reversed-phase chemically bonded non-porous (micropellicular) dp= 1.5/~m stationary phases. On these modern phases the time for analysis of complex mixtures of solutes -whether monomeric or polymeric (e. g. drugs, vitamins, peptides, or proteins) -is very short compared with that on porous phases. Different surface chemistries were elaborated for the separation of different types of sample. For the separation of small molecules a long-chain (C14) hydrocarbon-coated phase seems to be optimum; a short chain (C6) hydrocarbon bonded to the surface of the silica seems better for the separation of polymers. The efficiency, the low analysis times, and sensitivities were demonstrated by the separation of different proteins, peptides, drugs, alkaloids, and mixtures of waterand fat-soluble vitamins.
Key WordsColumn liquid chromatography Non-porous stationary phase Opium alkaloids Poppy, Papaver somniferum L.
SummaryA new reversed-phase (RP) HPLC method has been developed and validated for the separation of the main opium alkaloids morphine, codeine, thebaine, papaverine and noscapine on a non-porous (micropellicular) stationary phase. On this phase quantification of the compounds by internal standardization with brucine was achieved extremely rapidly, in ca 1.5 min, only. Thus, the analysis time for the opium alkaloids was approximately one tenth of that on porous stationary phases. Different opium samples were investigated using non-porous and porous packings. The correlation between the results was excellent.
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