Of the primary responses to contemporary climate change – “move, adapt, acclimate, or die” – that are available to organisms, “acclimate” may be effectively achieved through behavioral modification. Behavioral flexibility allows animals to rapidly cope with changing environmental conditions, and behavior represents an important component of a species’ adaptive capacity in the face of climate change. However, there is currently a lack of knowledge about the limits or constraints on behavioral responses to changing conditions. Here, we characterize the contexts in which organisms respond to climate variability through behavior. First, we quantify patterns in behavioral responses across taxa with respect to timescales, climatic stimuli, life‐history traits, and ecology. Next, we identify existing knowledge gaps, research biases, and other challenges. Finally, we discuss how conservation practitioners and resource managers can incorporate an improved understanding of behavioral flexibility into natural resource management and policy decisions.

Background: Distributional responses by alpine taxa to repeated, glacial-interglacial cycles throughout the last two million years have signi cantly in uenced the spatial genetic structure of populations. These effects have been exacerbated for the American pika (Ochotona princeps), a small alpine lagomorph constrained by thermal sensitivity and a limited dispersal capacity. As a species of conservation concern, long-term lack of gene ow has important consequences for landscape genetic structure and levels of diversity within populations. Here, we use reduced representation sequencing (ddRADseq) to provide a genome-wide perspective on patterns of genetic variation across pika populations representing distinct subspecies. To investigate how landscape and environmental features shape genetic variation, we collected genetic samples from distinct geographic regions as well as across ner spatial scales in two geographically proximate mountain ranges of eastern Nevada. Results: Our genome-wide analyses corroborate range-wide, mitochondrial subspeci c designations and reveal pronounced ne-scale population structure between the Ruby Mountains and East Humboldt Range of eastern Nevada. Populations in Nevada were characterized by low genetic diversity (=0.0006-0.0009; W =0.0005-0.0007) relative to populations in California (=0.0014-0.0019; W =0.0011-0.0017) and the Rocky Mountains (=0.0025-0.0027; W =0.0021-0.0024), indicating substantial genetic drift in these isolated populations. Tajima's D was positive for all sites (D=0.240-0.811), consistent with recent contraction in population sizes range-wide. Conclusions: Substantial in uences of geography, elevation and climate variables on genetic differentiation were also detected and may interact with the regional effects of anthropogenic climate change to force the loss of unique genetic lineages through continued population extirpations in the Great Basin and Sierra Nevada.

Background: Contemporary climate change is affecting nearly all biomes, causing shifts in animal distributions, phenology, and persistence. Favorable microclimates may buffer organisms against rapid changes in climate, thereby allowing time for populations to adapt. The degree to which microclimates facilitate the local persistence of climate-sensitive species, however, is largely an open question. We addressed the importance of microrefuges in mammalian thermal specialists, using the American pika (Ochotona princeps) as a model organism. Pikas are sensitive to ambient temperatures, and are active year-round in the alpine where conditions are highly variable. We tested four hypotheses about the relationship between microrefuges and pika occurrence: 1) Local-habitat Hypothesis (local-habitat conditions are paramount, regardless of microrefuge); 2) Surface-temperature Hypothesis (surrounding temperatures, unmoderated by microrefuge, best predict occurrence); 3) Interstitial-temperature Hypothesis (temperatures within microrefuges best predict occurrence), and 4) Microrefuge Hypothesis (the degree to which microrefuges moderate the surrounding temperature facilitates occurrence, regardless of other habitat characteristics). We examined pika occurrence at 146 sites across an elevational gradient. We quantified pika presence, physiographic habitat characteristics and forage availability at each site, and deployed paired temperature loggers at a subset of sites to measure surface and subterranean temperatures. Results: We found strong support for the Microrefuge Hypothesis. Pikas were more likely to occur at sites where the subsurface environment substantially moderated surface temperatures, especially during the warm season. Microrefugium was the strongest predictor of pika occurrence, independent of other critical habitat characteristics, such as forage availability. Conclusions: By modulating surface temperatures, microrefuges may strongly influence where temperature-limited animals persist in rapidly warming environments. As climate change continues to manifest, efforts to understand the changing dynamics of animal-habitat relationships will be enhanced by considering the quality of microrefuges.

The indirect effects of changing climate in modulating trophic interactions can be as important as the direct effects of climate stressors on consumers. The success of the herbivorous juvenile stage of the crown-of-thorns starfish (COTS), may be affected by the impacts of ocean conditions on its crustose coralline algal (CCA) food. To partition the direct effects of near future ocean acidification on juvenile COTS and indirect effects through changes in their CCA food, COTS were grown in three pH levels (7.9, 7.8, 7.6) and fed CCA grown at similar pH levels. Consumption of CCA by COTS was bolstered when the COTS were grown in low pH and when they were fed CCA grown in low pH regardless of the pH in which the COTS were reared. COTS fed CCA grown at pH 7.6 grew fastest, but the pH/CO that the COTS were reared in had no direct effect on growth. Ocean acidification conditions decreased the C : N ratio and carbonate levels in the CCA. Bolstered growth in COTS may be driven by enhanced palatability, increased nutritive state and reduced defences of their CCA food. These results indicate that near future acidification will increase the success of early juvenile COTS and boost recruitment into the coral-eating life stage.

Collisions between wildlife and vehicles are detrimental to both wildlife and human safety. A variety of mitigation methods have been deployed with the intent of increasing ungulate awareness of approaching vehicles. Wildlife warning reflectors are one such method. Roadside reflectors are designed to reflect headlights into the right-of-way and alert ungulates to approaching vehicles. Studies of the effectiveness of these reflectors have yielded mixed results. We conducted a robust test of reflectors in central Wyoming, USA, during 2013 and 2014, and unexpectedly discovered a potentially more effective method than the reflectors for reducing collisions between vehicles and mule deer (Odocoileus hemionus). In our initial experiment, we manipulated 10 1.6-km segments of highway by leaving their reflectors exposed or covering them with a white canvas bag with the intention of neutralizing the reflector. The treatment configuration was swapped monthly. We counted deer carcasses under each treatment for 1 year and observed deer roadcrossing behavior using thermal video cameras. Carcass rates were 33% less in the white canvas treatment relative to uncovered reflectors. Deer in the white canvas treatment also stopped before entering the road 20% more often, ran into the road from the right-of-way 11% less often, and fled from the road 12% more often than when reflectors were uncovered. In a follow-up experiment, we found that deer carcass rates were 32% less when reflectors were exposed versus covered with black canvas. We further found that deer road-crossing behavior was least risky in a white canvas treatment, intermediate in a reflectors treatment, and most risky when reflectors were removed from posts. Taken together, these results indicate that, although reflectors were moderately effective, white canvas was substantially more effective in reducing deer-vehicle collisions. This unexpected finding suggests that new vigilance-enhancing mitigation methods should be explored as a way to reduce wildlife-vehicle collisions. Ó

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Contemporary climate change affects nearly all biomes, causing shifts in animal distributions and resource availability. Changes in resource selection may allow individuals to offset climatic stress, thereby providing a mechanism for persistence amidst warming conditions. Whereas the role of predation risk in food choice has been studied broadly, the extent to which individuals respond to thermoregulatory risk by changing resource preferences is unclear. We addressed whether individuals compensated for temperature-related reductions in foraging time by altering forage preferences, using the American pika (Ochotona princeps) as a model species. We tested two hypotheses: (1) food-quality hypothesis-individuals exposed to temperature extremes should select higher-quality vegetation in return for accepting a physiologically riskier feeding situation; and (2) food-availability hypothesis-individuals exposed to temperature extremes should prioritize foraging quickly, thereby decreasing selection for higher-quality food. We quantified the composition and quality (% moisture, % nitrogen, and fiber content) of available and harvested vegetation, and deployed a network of temperature sensors to measure in situ conditions for 30 individuals, during July-Sept., 2015. Individuals exposed to more extreme daytime temperatures showed increased selection for high-nitrogen and for low-fiber vegetation, demonstrating strong support for the food-quality hypothesis. By contrast, pikas that experienced warmer conditions did not reduce selection for any of the three vegetation-quality metrics, as predicted by the food-availability hypothesis. By shifting resource-selection patterns, temperature-limited animals may be able to proximately buffer some of the negative effects associated with rapidly warming environments, provided that sufficient resources remain on the landscape.

Contemporary climate change is altering temperature profiles across the globe. Increasing temperatures can reduce the amount of time during which conditions are suitable for animals to engage in essential activities, such as securing food. Behavioural plasticity, the ability to alter behaviour in response to the environment, may provide animals with a tool to adjust to changes in the availability of suitable thermal conditions. The extent to which individuals can alter fitness‐enhancing behaviours, such as food collection, to proximately buffer variation in temperature, however, remains unclear. Even less well understood are the potential performance advantages of flexible strategies among endotherms. We examined the degree to which individuals altered rates of food collection in response to temperature, and two potential benefits, using the American pika (Ochotona princeps), a temperature‐sensitive, food‐hoarding mammal, as a model. From July–September 2013–2015, we used motion‐activated cameras and in situ temperature loggers to examine pika food‐caching activity for 72 individuals across 10 sites in the central Rocky Mountains, USA. We quantified % nitrogen by cache volume as a metric of cache quality, and the number of events during which pikas were active in temperatures ≥25°C as a measure of potential thermoregulatory stress. We found a strong negative effect of temperature on the rate at which pikas cached food. Individual responses to temperature varied substantially in both the level of food‐collecting activity and in the degree to which individuals shifted activity with warming temperature. After accounting for available foraging time, individuals that exhibited greater plasticity collected a comparable amount of nitrogen, while simultaneously experiencing fewer occasions in which temperatures eclipsed estimated thermal tolerances. By varying food‐collection norms of reaction, individuals were able to plastically respond to temperature‐driven reductions in foraging time. Through this increased flexibility, individuals amassed food caches of comparable quality, while minimizing exposure to potentially stressful thermal conditions. Our results suggest that, given sufficient resource quality and availability, plasticity in foraging activity may help temperature‐limited endotherms adjust to climate‐related constraints on foraging time.