Confocal Raman microscopy, a non-invasive, label-free, and high spatial resolution imaging technique is employed to trace the anticancer drug paclitaxel in living Michigan Cancer Foundation-7 (MCF-7) cells. The Raman images were treated by K-mean cluster analysis to detect the drug in cells. Distribution of paclitaxel in cells is verified by calculating the correlation coefficient between the reference spectrum of the drug and the whole Raman image spectra. A time dependent gradual diffusion of paclitaxel all over the cell is observed suggesting a complementary picture of the pharmaceutical action of this drug based on rapid binding of free tubulin to crystallized paclitaxel.
Abstract:This study investigated degrees of conversion of oxygen inhibited layer (OIL) of organic dental resins for restoration using Confocal-Raman spectroscopy. The aim was to determine which laser is adapted to determine the degrees of conversion of OIL and to measure variations of thickness and degrees of conversion in OIL with respect to monomers proportions. Bis-GMA (bis-phenol A glycidyl dimethacrylate) and TEGDMA (triethylene glycol dimethacrylate) based resins with various ratio of an equimolecular mixture of camphorquinone/EDMAB (ethyl (4-dimethyl amino) benzoate) were studied with different lasers by confocalRaman spectroscopy. Results show that this technique is adapted for the non destructive measurement of OIL. The Thickness of OIL is not correlated with the proportions of Bis-GMA and TEGDMA in the resin and was close to 3-4µm. Thickness of OIL is very thin without inorganic fillers (3 or 4 µm). Inorganic fillers might be responsible of greater OIL in composite resins.
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