Raman confocal microscopy and AFM combined studies of cancerous cells treated with Paclitaxel

Derely, L.; Collart-Dutilleul, Pierre-Yves; Welle, Sylvain Michotte de; Szabo, Vivien; Gergely, Csilla; Cuisinier, Frédéric

doi:10.1117/12.874893

Cited by 5 publications

(2 citation statements)

References 14 publications

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“…2,3 The Raman spectra of cells contain the fingerprints of all molecules present, so they are very complex. Individual cells' Raman spectra 4,5 and the localization of intracellular nanoparticles [6][7][8] have been reported previously. High spatial resolution and a unique compositional sensitivity are the main advantages of confocal Raman microscopy over other spectroscopic methods.…”

Section: Introductionmentioning

confidence: 99%

Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel

et al. 2013

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Abstract. Confocal Raman microscopy is a noninvasive, label-free imaging technique used to study apoptosis of live MCF-7 cells. The images are based on Raman spectra of cells components, and their apoptosis is monitored through diffusion of cytochrome c in cytoplasm. K-mean clustering is used to identify mitochondria in cells, and correlation analysis provides the cytochrome c distribution inside the cells. Our results demonstrate that incubation of cells for 3 h with 10 μM of paclitaxel does not induce apoptosis in MCF-7 cells. On the contrary, incubation for 30 min at a higher concentration (100 μM) of paclitaxel induces gradual release of the cytochrome c into the cytoplasm, indicating cell apoptosis via a caspase independent pathway.

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Section: Introductionmentioning

confidence: 99%

Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel

et al. 2013

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“…[10][11][12][13][14] Raman spectra of cells are very complex because they contain the fingerprints of all molecules present. Raman spectral maps of individual cells [15][16][17][18] and localization of intracellular nanoparticles 12,19,20 have been achieved. The main advantages of confocal Raman microscopy include high spatial resolution and a unique compositional sensitivity intrinsic to spectroscopic methods.…”

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confidence: 99%

Label-free detection of anticancer drug paclitaxel in living cells by confocal Raman microscopy

Salehi¹,

Derely²,

Végh³

et al. 2013

Applied Physics Letters

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Confocal Raman microscopy, a non-invasive, label-free, and high spatial resolution imaging technique is employed to trace the anticancer drug paclitaxel in living Michigan Cancer Foundation-7 (MCF-7) cells. The Raman images were treated by K-mean cluster analysis to detect the drug in cells. Distribution of paclitaxel in cells is verified by calculating the correlation coefficient between the reference spectrum of the drug and the whole Raman image spectra. A time dependent gradual diffusion of paclitaxel all over the cell is observed suggesting a complementary picture of the pharmaceutical action of this drug based on rapid binding of free tubulin to crystallized paclitaxel.

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Spectroscopic detection of chemotherapeutics and antioxidants

et al. 2012

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Raman confocal microscopy and AFM combined studies of cancerous cells treated with Paclitaxel

Cited by 5 publications

References 14 publications

Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel

Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel

Label-free detection of anticancer drug paclitaxel in living cells by confocal Raman microscopy

Spectroscopic detection of chemotherapeutics and antioxidants

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