In an arrhenoblastoma of a 24 years old female patient the enzyme activities of the intermediary carbonhydrate metabolism, the citric acid cycle, the glycerophosphate cycle, the pentose-phosphate shunt as well as the steroid-dehydrogenases were measured quantitatively and localized histochemically. The striking high activities of the glycolytic enzymes, the presence of steroid-dehydrogenases and the only moderately increased enzyme activities of the citric acid cycle in comparison with the stroma ovarii identify the arrhenoblastoma as a tissue with steroid hormonal metabolism. High activities of the mentioned enzymes and especially the activities of the steroid-dehydrogenases were localized histochemically only in Leydig-cell-like cells. Therefore these cells may be considered as structures of steroid-biogenesis. The enzyme activity pattern of the steroiddehydrogenases illuminates the biogenetic pathways of androgens. The low activities of the 17β-hydroxysteroid-dehydrogenase in this tissue in comparison with other steroid producing tissues indicate a special testosterone metabolism.
In the bovine corpora lutea periodical activities of the Δ53β-, 3β-, 17β-and 20β-hydroxysteroid dehydrogenase (OHSDH) as well as activities of glyceraldehyde-3-phosphate- and glucose-6-phosphate dehydrogenase were measured quantitatively and the alterations throughout the different stages of the cycle were studied. After homogenization of the tissue and fractionate centrifugation the enzyme activities were determined by a standardized optical method. The activities of the Δ53β-, and 3β- and 17β-OHSDH increase slowly during the first 7 days of the cycle, the maximum is reached abruptly on the 12th to 13th day of the cycle. After a striking reduction the activities decline continually to the 19th to 21st day reaching the values detected at the beginning of the cycle. The 20β-OHSDH increases slowly to the maximum on the 15th day of the cycle. Activities of the 3α-OHSDH were obtained only inconsistently. The behaviour of the activities of G6PDH was nearly identical with that of the 3β-OHSDH, while the GAPDH shows only little fluctuations of its activities. The obtained enzyme activity patterns of the maturating and high functional corpus luteum correspond to the well-known data of the biosynthetic function of the bovine corpus luteum. The changes of the amounts of progesterone and 20β-progesterol agree with the course of the activities of the 3β- resp. 20β-OHSDH.
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