Compared to the overall multiplicity of more than 20 plant Hsfs, detailed analyses are mainly restricted to tomato and Arabidopsis and to three important representatives of the family (Hsfs A1, A2 and B1). The three Hsfs represent examples of striking functional diversification specialized for the three phases of the heat stress (hs) response (triggering, maintenance and recovery). This is best illustrated for the tomato Hsf system: (i) HsfA1a is the master regulator responsible for hs-induced gene expression including synthesis of HsfA2 and HsfB1. It is indispensible for the development of thermotolerance. (ii) Although functionally equivalent to HsfA1a, HsfA2 is exclusively found after hs induction and represents the dominant Hsf, the "working horse" of the hs response in plants subjected to repeated cycles of hs and recovery in a hot summer period. Tomato HsfA2 is tightly integrated into a network of interacting proteins (HsfA1a, Hsp17-CII, Hsp17-CI) influencing its activity and intracellular distribution. (iii) Because of structural peculiarities, HsfB1 acts as coregulator enhancing the activity of HsfA1a and/or HsfA2. But in addition, it cooperates with yet to be identified other transcription factors in maintaining and/or restoring housekeeping gene expression.

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The AcrySof-acrylic IOL was less susceptible to primary adherence and 18 hour biofilm formation by P. aeruginosa than the PMMA and silicone IOLs, indicating that this material reduced pseudomonad adherence and the risk of endophthalmitis following cataract surgery.

The method provided a quantitative basis for the clinical classification of viscoelastic materials as cohesive or dispersive. The aspiration kinetics profile (curve shape), maximum rate of removal, and vacuum levels at which the bolus removal of the viscoelastic agent started (break point) were useful in characterizing the viscoelastic agents. Because the results agree with the clinical impression of cohesion/dispersion, this method may be used to predict the surgical performance of viscoelastic agents.

The results of this experimental model suggest that cohesive viscoelastics are readily removed from the cells, while dispersive viscoelastics are highly retained. In addition, mutual surface interaction (electrical charge and other properties) plays a significant role in determining the retention of viscoelastics on the corneal endothelial cell surface following I/A.

This study utilized peroxidase cytochemical methods to survey and analyze the distribution, morphology, and functional relationship of the various components of sensory nerve endings in a focal region of the anterior hard palate (incisive papilla) of the rat. The studies included an anatomical survey of this relatively unknown oral structure, confirmation of the trigeminal origin (labeled by retrograde axonal transport of WGA-HRP injected into the incisive papilla) of the sensory nerve endings, and a complete analysis of the distribution of these nerve endings (labeled by anterograde axonal transport of HRP or WGA-HRP injected into the trigeminal ganglion). Three methods of fixation and two methods of cytochemical reaction were used for selection of an optimal technique for these studies. The results showed distinct patterns of peroxidase-labeled sensory nerve endings at three regions of the incisive papilla: a) dome region (ventral), where labeled nerve endings formed three parallel channels in association with three surface ridges; b) chemosensory corpuscle enriched region (medial to incisive canal), where 82% of the 30-40 chemosensory corpuscles were labeled; and c) lateral labium (lateral to incisive canal), where labeled nerve endings formed a circumscribed network guarding the orifice of incisive canal. The discrete organization of multiple sensory nerve endings in the incisive papilla of the rat may provide an easily accessible model system for various studies in sensory physiology.

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