No abstract
Penelitian bertujuan menguji komponen kualitas dari beberapa klon kentang hasil seleksi untuk keripik. Penelitian dilakukan mulai bulan Juli sampai dengan September 2010 menggunakan metode eksperimen di laboratorium. Rancangan yang digunakan ialah acak kelompok dengan tiga ulangan. Perlakuan yang diuji terdiri atas 10 klon kentang yaitu (1) 385524.9 x 392639.34, (2) 393077.54 x 391011.17, (3) 393077.54 x 391011.17, (4) 391011.17 x 391580.30, (5) 391011.17 x 385524.9, (6) 393077.54 x 391011.17, (7) 391011.17 x 385524.9, (8) 391011.17 x 385524.9, (9) 393033.54 x 391580.30, dan (10) Granola (kontrol). Hasil uji organoleptik menunjukkan bahwa keripik kentang yang memiliki skor antara 2,00-2,36 (kuning merata) untuk chips kentang ialah klon 7 (391011.17 x 385524.9) dan klon 8 (391011.17 x 385524.9). Kandungan gula reduksi dari kedua klon tersebut, yaitu masing-masing 0,029 dan 0,023% lebih rendah daripada kandungan gula reduksi pada klon-klon lainnya yang keripiknya berwarna gelap. Klon-klon tersebut memenuhi persyaratan kualitas dan berpeluang untuk digunakan sebagai bahan baku industri pengolahan keripik kentang. <br /><br /><br />The objective of the research was to determine the quality of potato clones resulted from selection for potato chips. Quality test of 10 selective clones was determined. The research was conducted from July to September 2010, and was arranged in a completely randomized block design with three replications. Treatments consisted of (1) 385524.9 x 392639.34, (2) 393077.54 x 391011.17, (3) 393077.54 x 391011.17, (4) 391011.17 x 391580.30, (5) 391011.17 x 385524.9, (6) 393077.54 x 391011.17, (7) 391011.17 x 385524.9, (8) 391011.17 x 385524.9, (9) 393033.54 x 391580.30, and (10) Granola (control). The results showed that chips which had a score value between 2.00 to 2.36 (yellow uniform) for potato chips were clone 7 (391011.17 x 385524.9) and clone 8 (391011.17 x 385524.9). The reducted sugar content of these clones was lower (0.029 and 0.023% respectively) than the reducted sugar content of the other potato clones which had dark color. The potato clones had good quality and fulfilled conditions for potato chips processing.
Populations of P. infestans are dynamic, with mutation, migration, sexual reproduction, and host having contributed to the evolution of new clonal lineages or genotypes with different epidemiological, genotypic, and phenotypic characteristics (Goodwin, 1997; Hu et al., 2012). Globalization has increased the trade in agricultural commodities such as potato between countries. Potato tubers intended for processing or for use as seed are imported or exported from one country to another based on supply and demand. P. infestans remains dormant (latent) in seed tubers at low temperatures (4°C). As such, symptomless seed tubers are easily overlooked in intercontinental shipments (Johnson and Cummings, 2009). The introduction of new genotypes in seed tubers has resulted in changes in local population compositions of
The phenotypic performances have formed the basis for genetic purity evaluation. However, the phenotypic based the genetic purity assessment are time-consuming, expensive, and not stable due to a great environmental effect on traits expression. While, the genotypic based genetic purity evaluation of a variety offers an efficient, more stable, and precise result than those phenotypic method due to no environmental factors involved. The objectives of this study were to determine genetic purity and population structure of Granola L. potato variety derived from twelve collection sources using microsatellite markers. This study consisted of following steps, such as cultivation of Granola L. potato varieties in the field, DNA isolation, PCR analysis, amplified DNA fragment analysis. The results showed that Granola L. variety derived from twelve sources had high level of genetic purity which indicated by genetic similarity value of 0.96 observed in present study. Based on population structure analysis, Granola L. variety might have consisted of two sub-populations of which one sub-population was more dominant than other sub-population. Therefore, genetic purification activities for Granola L. variety are still needed to increase genetic purity and to reduce phenotypic differences in future.
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